Unravelling the shape and structural assembly of the photosynthetic GAPDH-CP12-PRK complex from Arabidopsis thaliana by small-angle X-ray scattering analysis.

IF 2.2 4区生物学

Acta Crystallographica Section D: Biological Crystallography Pub Date : 2015-12-01 DOI:10.1107/S1399004715018520

Alessandra Del Giudice, N. Pavel, L. Galantini, G. Falini, P. Trost, S. Fermani, F. Sparla

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引用次数: 13

Abstract

Oxygenic photosynthetic organisms produce sugars through the Calvin-Benson cycle, a metabolism that is tightly linked to the light reactions of photosynthesis and is regulated by different mechanisms, including the formation of protein complexes. Two enzymes of the cycle, glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and phosphoribulokinase (PRK), form a supramolecular complex with the regulatory protein CP12 with the formula (GAPDH-CP122-PRK)2, in which both enzyme activities are transiently inhibited during the night. Small-angle X-ray scattering analysis performed on both the GAPDH-CP12-PRK complex and its components, GAPDH-CP12 and PRK, from Arabidopsis thaliana showed that (i) PRK has an elongated, bent and screwed shape, (ii) the oxidized N-terminal region of CP12 that is not embedded in the GAPDH-CP12 complex prefers a compact conformation and (iii) the interaction of PRK with the N-terminal region of CP12 favours the approach of two GAPDH tetramers. The interaction between the GAPDH tetramers may contribute to the overall stabilization of the GAPDH-CP12-PRK complex, the structure of which is presented here for the first time.

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利用小角度x射线散射分析揭示拟南芥光合作用GAPDH-CP12-PRK复合物的形状和结构组装。

含氧光合生物通过卡尔文-本森循环产生糖，这是一种与光合作用的光反应密切相关的代谢，受到不同机制的调节，包括蛋白质复合物的形成。该循环的两种酶，甘油醛-3-磷酸脱氢酶(GAPDH)和磷酸脲激酶(PRK)，与调节蛋白CP12形成超分子复合物，分子式为(GAPDH- cp122 -PRK)2，这两种酶的活性在夜间被短暂抑制。对拟南芥GAPDH-CP12-PRK复合物及其组分GAPDH-CP12和PRK进行的小角度x射线散射分析表明:(i) PRK具有细长、弯曲和螺旋形状，(ii)未嵌入GAPDH-CP12复合物的CP12的氧化n端区域倾向于紧凑的构象，(iii) PRK与CP12 n端区域的相互作用有利于两种GAPDH四聚体的接近。GAPDH四聚体之间的相互作用可能有助于GAPDH- cp12 - prk复合物的整体稳定，该复合物的结构在这里首次被提出。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Acta Crystallographica Section D: Biological Crystallography 生物-晶体学

自引率

13.60%

发文量

审稿时长

3 months

期刊介绍： Acta Crystallographica Section D welcomes the submission of articles covering any aspect of structural biology, with a particular emphasis on the structures of biological macromolecules or the methods used to determine them. Reports on new structures of biological importance may address the smallest macromolecules to the largest complex molecular machines. These structures may have been determined using any structural biology technique including crystallography, NMR, cryoEM and/or other techniques. The key criterion is that such articles must present significant new insights into biological, chemical or medical sciences. The inclusion of complementary data that support the conclusions drawn from the structural studies (such as binding studies, mass spectrometry, enzyme assays, or analysis of mutants or other modified forms of biological macromolecule) is encouraged. Methods articles may include new approaches to any aspect of biological structure determination or structure analysis but will only be accepted where they focus on new methods that are demonstrated to be of general applicability and importance to structural biology. Articles describing particularly difficult problems in structural biology are also welcomed, if the analysis would provide useful insights to others facing similar problems.