Instant formation of horizontally ordered nanofibrous hydrogel films and direct investigation of peculiar neuronal cell behaviors atop.

IF 11.3 1区 医学 Q1 Medicine
Jaeil Park, Thi Thuy Chau Nguyen, Su-Jin Lee, Sungrok Wang, Dongmi Heo, Dong-Hee Kang, Alexander Tipan-Quishpe, Won-June Lee, Jongwon Lee, Sung Yun Yang, Myung-Han Yoon
{"title":"Instant formation of horizontally ordered nanofibrous hydrogel films and direct investigation of peculiar neuronal cell behaviors atop.","authors":"Jaeil Park,&nbsp;Thi Thuy Chau Nguyen,&nbsp;Su-Jin Lee,&nbsp;Sungrok Wang,&nbsp;Dongmi Heo,&nbsp;Dong-Hee Kang,&nbsp;Alexander Tipan-Quishpe,&nbsp;Won-June Lee,&nbsp;Jongwon Lee,&nbsp;Sung Yun Yang,&nbsp;Myung-Han Yoon","doi":"10.1186/s40824-023-00344-3","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Hydrogels have been widely used in many research fields owing to optical transparency, good biocompatibility, tunable mechanical properties, etc. Unlike typical hydrogels in the form of an unstructured bulk material, we developed aqueous dispersions of fiber-shaped hydrogel structures with high stability under ambient conditions and their application to various types of transparent soft cell culture interfaces with anisotropic nanoscale topography.</p><p><strong>Method: </strong>Nanofibers based on the polyvinyl alcohol and polyacrylic acid mixture were prepared by electrospinning and hydrogelified to nano-fibrous hydrogels (nFHs) after thermal crosslinking and sulfuric acid treatment. By modifying various material surfaces with positively-charged polymers, negatively-charged superabsorbent nFHs could be selectively patterned by employing micro-contact printing or horizontally aligned by applying shear force with a wired bar coater.</p><p><strong>Results: </strong>The angular distribution of bar-coated nFHs was dramatically reduced to ± 20° along the applied shear direction unlike the drop-coated nFHs which exhibit random orientations. Next, various types of cells were cultured on top of transparent soft nFHs which showed good viability and attachment while their behaviors could be easily monitored by both upright and inverted optical microscopy. Particularly, neuronal lineage cells such as PC 12 cells and embryonic hippocampal neurons showed highly stretched morphology along the overall fiber orientation with aspect ratios ranging from 1 to 14. Furthermore, the resultant neurite outgrowth and migration behaviors could be effectively controlled by the horizontal orientation and the three-dimensional arrangement of underlying nFHs, respectively.</p><p><strong>Conclusion: </strong>We expect that surface modifications with transparent soft nFHs will be beneficial for various biological/biomedical studies such as fundamental cellular studies, neuronal/stem cell and/or organoid cultures, implantable probe/device coatings, etc.</p>","PeriodicalId":9079,"journal":{"name":"Biomaterials Research","volume":null,"pages":null},"PeriodicalIF":11.3000,"publicationDate":"2023-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10009932/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Biomaterials Research","FirstCategoryId":"5","ListUrlMain":"https://doi.org/10.1186/s40824-023-00344-3","RegionNum":1,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}
引用次数: 0

Abstract

Background: Hydrogels have been widely used in many research fields owing to optical transparency, good biocompatibility, tunable mechanical properties, etc. Unlike typical hydrogels in the form of an unstructured bulk material, we developed aqueous dispersions of fiber-shaped hydrogel structures with high stability under ambient conditions and their application to various types of transparent soft cell culture interfaces with anisotropic nanoscale topography.

Method: Nanofibers based on the polyvinyl alcohol and polyacrylic acid mixture were prepared by electrospinning and hydrogelified to nano-fibrous hydrogels (nFHs) after thermal crosslinking and sulfuric acid treatment. By modifying various material surfaces with positively-charged polymers, negatively-charged superabsorbent nFHs could be selectively patterned by employing micro-contact printing or horizontally aligned by applying shear force with a wired bar coater.

Results: The angular distribution of bar-coated nFHs was dramatically reduced to ± 20° along the applied shear direction unlike the drop-coated nFHs which exhibit random orientations. Next, various types of cells were cultured on top of transparent soft nFHs which showed good viability and attachment while their behaviors could be easily monitored by both upright and inverted optical microscopy. Particularly, neuronal lineage cells such as PC 12 cells and embryonic hippocampal neurons showed highly stretched morphology along the overall fiber orientation with aspect ratios ranging from 1 to 14. Furthermore, the resultant neurite outgrowth and migration behaviors could be effectively controlled by the horizontal orientation and the three-dimensional arrangement of underlying nFHs, respectively.

Conclusion: We expect that surface modifications with transparent soft nFHs will be beneficial for various biological/biomedical studies such as fundamental cellular studies, neuronal/stem cell and/or organoid cultures, implantable probe/device coatings, etc.

Abstract Image

Abstract Image

Abstract Image

水平有序纳米纤维水凝胶薄膜的即时形成和对顶层特殊神经细胞行为的直接研究。
背景:水凝胶具有光学透明性、良好的生物相容性、可调节的力学性能等优点,被广泛应用于许多研究领域。与典型的非结构体材料形式的水凝胶不同,我们开发了在环境条件下具有高稳定性的纤维状水凝胶结构的水分散体,并将其应用于具有各向异性纳米级形貌的各种类型的透明软细胞培养界面。方法:以聚乙烯醇和聚丙烯酸为基料,采用静电纺丝法制备纳米纤维,经热交联和硫酸处理后水解成纳米纤维水凝胶(nFHs)。通过用带正电荷的聚合物修饰各种材料表面,带负电荷的高吸水性nfh可以通过采用微接触印刷来选择性地图案化,或者通过使用线材涂布机施加剪切力来水平对齐。结果:棒状包覆的nFHs沿施加剪切方向的角分布明显减小到±20°,而滴状包覆的nFHs则表现为随机方向。然后,在透明软nFHs上培养各种类型的细胞,这些细胞表现出良好的活力和附着性,并且在直立和倒置光学显微镜下都易于监测其行为。特别是,神经元谱系细胞,如pc12细胞和胚胎海马神经元,沿整个纤维方向表现出高度拉伸的形态,纵横比在1到14之间。此外,神经突的生长和迁移行为可以分别由下垫nfh的水平取向和三维排列有效控制。结论:我们期望透明软nFHs的表面修饰将有利于各种生物/生物医学研究,如基础细胞研究、神经元/干细胞和/或类器官培养、植入式探针/设备涂层等。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Biomaterials Research
Biomaterials Research Medicine-Medicine (miscellaneous)
CiteScore
10.20
自引率
3.50%
发文量
63
审稿时长
30 days
期刊介绍: Biomaterials Research, the official journal of the Korean Society for Biomaterials, is an open-access interdisciplinary publication that focuses on all aspects of biomaterials research. The journal covers a wide range of topics including novel biomaterials, advanced techniques for biomaterial synthesis and fabrication, and their application in biomedical fields. Specific areas of interest include functional biomaterials, drug and gene delivery systems, tissue engineering, nanomedicine, nano/micro-biotechnology, bio-imaging, regenerative medicine, medical devices, 3D printing, and stem cell research. By exploring these research areas, Biomaterials Research aims to provide valuable insights and promote advancements in the biomaterials field.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信