Identification of Novel miRNAs Involved in Cancer Progression and Metastasis in Clear Cell Renal Cell Carcinoma

Q3 Mathematics
R. H. Hamoode, T. A. Rafaa, R. H. Abduljaleel, A. Suleiman
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引用次数: 0

Abstract

MicroRNAs are non-coding molecules 21–23 nucleotides long that are involved in post-transcriptional regulation of gene expression by mRNA silencing. There is evidence that miRNA activity is associated with various types of human cancer, such as breast cancer, colorectal cancer, ovarian cancer and hepatocellular carcinoma, as well as with clear cell renal cell carcinoma. In 2020, the incidence of kidney cancer worldwide was 319,016. Clear cell renal cell carcinoma is associated with loss or mutation of the VHL gene. The aim of this study was to identify miRNAs that are expressed differently in samples of normal kidney tissue and tissue affected by clear cell renal cell carcinoma. For this study, a public miRNA dataset from ArrayExpress was obtained and post-processed. Mapping, identification of known and new microRNAs, and quantification were performed using the miRDeep2 computer program from the DESeq R/Bioconductor package. We performed target identification and functional load analysis using GeneCodis4. A total of 2656 miRNAs were found to be differentially expressed, of which 229 miRNAs were overexpressed and 302 were underexpressed. In this study, we identified five miRNAs that were already known from the literature to be significantly associated with clear cell renal cell carcinoma. In addition, we found a set of five microRNAs that had not previously associated with clear cell renal cell carcinoma.
透明细胞肾细胞癌中参与肿瘤进展和转移的新型mirna的鉴定
microrna是非编码分子,长21-23个核苷酸,通过mRNA沉默参与转录后基因表达调控。有证据表明,miRNA活性与多种类型的人类癌症有关,如乳腺癌、结直肠癌、卵巢癌和肝细胞癌,以及透明细胞肾细胞癌。2020年,全球肾癌发病率为319016例。透明细胞肾细胞癌与VHL基因的缺失或突变有关。本研究的目的是鉴定在正常肾组织和透明细胞肾细胞癌组织样本中表达不同的mirna。在这项研究中,从ArrayExpress获得了一个公开的miRNA数据集并进行了后处理。使用DESeq R/Bioconductor软件包中的miRDeep2计算机程序对已知和新的microrna进行制图、鉴定和定量。我们使用GeneCodis4进行了目标识别和功能负载分析。共发现2656个mirna差异表达,其中229个mirna过表达,302个mirna过表达。在这项研究中,我们从文献中发现了五种与透明细胞肾细胞癌显著相关的mirna。此外,我们还发现了一组5个microrna,这些microrna以前与透明细胞肾细胞癌没有关联。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Mathematical Biology and Bioinformatics
Mathematical Biology and Bioinformatics Mathematics-Applied Mathematics
CiteScore
1.10
自引率
0.00%
发文量
13
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