Toxocara Species Infection in Pet Dogs and Cats Ensured by Loop-Mediated Isothermal Amplification Assay

International Journal of Enteric Pathogens Pub Date : 2021-08-29 DOI:10.34172/ijep.2021.21

Hamid Hosseini, M. Zibaei, A. Miahipour, Z. Hatami, F. Firoozeh, Milad Badri

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Abstract

Background: Toxocariasis is a parasitic disease with global distribution. Many paratenic or transport hosts have been identified as the sources of infection, including vertebrates such as birds and humans. The definitive hosts of Toxocara are dogs and cats whose parasites live in their guts. Hence, the diagnosis of the disease in these animals is important. Objective: The present study was performed using the loop-mediated isothermal amplification (LAMP) method to diagnose toxocariasis. Materials and Methods: In the present study, 60 samples of pet feces were collected and subsequently evaluated by direct smear, formalin-ether, and LAMP. Results: Toxocara infection in dogs by microscopic and formal ether methods and LAMP method was 10% and 13.3%, respectively, while feline infection with this parasite was not observed either microscopically or molecularly. Conclusion: The present findings revealed that pet dogs can be considered as a source of infection for humans. Therefore, it is recommended that animal owners receive the necessary training to prevent the risks of transmitting zoonotic diseases.

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用环介导的等温扩增法检测宠物狗和猫的弓形虫感染

背景:弓形虫病是一种全球分布的寄生虫病。已确定许多副宿主或运输宿主是感染源，包括脊椎动物，如鸟类和人类。弓形虫的最终宿主是狗和猫，它们的寄生虫生活在它们的内脏里。因此，对这些动物的疾病诊断是重要的。目的:应用环介导等温扩增(LAMP)方法诊断弓形虫病。材料与方法:收集60份宠物粪便标本，采用直接涂片法、福尔马林醚法和LAMP法进行评价。结果:显微镜法、形式乙醚法和LAMP法检测犬弓形虫感染率分别为10%和13.3%，而猫的弓形虫感染在显微镜和分子上均未观察到。结论:目前的研究结果表明，宠物狗可以被认为是人类的感染源。因此，建议动物主人接受必要的培训，以防止传播人畜共患疾病的风险。

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来源期刊

International Journal of Enteric Pathogens

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审稿时长

28 weeks