Determination of Bisphenol in Indoor Dust Samples by Liquid Chromatography in Combination with Quadrupole Mass Spectrometry (LC-MS/MS)

Abstract

This study has focused on the development of the method LC-MS/MS to determine 2,2-bis(4-hydroxyphenyl)propane (bisphenol A: BPA) and 4,4’-dihydroxydiphenylmethane (bisphenol F: BPF) in indoor samples by using ultrasonication extraction and solid-phase extraction for preparation. The chromatographic procedure was performed on Kinetex C18 2.1 x 150 mm, 2.6 µm column with mobile phases: 2 mM HCOONH4 in MeOH and H2O:MeOH (90:10,…). The method quantification limits (MQL) of BPA and BPF were 15.0 and 12.0 ng/g, respectively. The mean recoveries were 80.4% for BPA and 93.8% for BPF (RSD<15%). The calibration curve for both BPA and BPF was linear over a concentration range of 1.0 to 500 ng/mL (R2 ≥ 0.999). Twenty-one samples were collected from private houses, laboratories, drug stores, and plastic recycling factories in Hanoi. BPA and BPF were found in dust samples with frequencies of 85.7 and 100%, respectively. Concentrations of BPA and BPF in dust samples were in the ranges of

分享

查看原文 本刊更多论文

液相色谱-四极杆质谱(LC-MS/MS)联合测定室内粉尘样品中的双酚

本研究主要建立了液相色谱-质谱联用技术(LC-MS/MS)，通过超声提取和固相萃取制备，测定室内样品中的2,2-双(4-羟基苯基)丙烷(双酚A: BPA)和4,4 ' -二羟基二苯基甲烷(双酚F: BPF)。色谱采用Kinetex C18 2.1 x 150 mm, 2.6µm色谱柱，流动相:2mm HCOONH4 in MeOH和H2O:MeOH(90:10，…)。双酚a和双酚f的定量限(MQL)分别为15.0和12.0 ng/g。双酚a的平均回收率为80.4%，双酚f的平均回收率为93.8% (RSD<15%)。BPA和BPF在1.0 ~ 500 ng/mL范围内均呈线性关系(R2≥0.999)。从河内的私人住宅、实验室、药店和塑料回收厂收集了21个样本。BPA和BPF在粉尘样品中的检测频率分别为85.7和100%。粉尘样品中BPA和BPF的浓度分别在< MQL-2230 ng/g(平均值:626)和< mql - 149000 ng/g(平均值:14700)范围内。根据测量的浓度，估计了不同年龄组的人通过室内灰尘摄入双酚的暴露量。在塑料回收工厂工作的人的估计接触剂量最高(23.6纳克/公斤-体重/天)，但低于EPA和欧洲食品安全局的口服参考剂量(50微克/公斤-体重/天)。

本文章由计算机程序翻译，如有差异，请以英文原文为准。