Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry-based biotyping of silk. I. Method development

H. Kajiwara, R. Murakami, K. Nakajima
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Abstract

SUMMARY A method for the analysis of silkworm ( Bombyx mori L.) cocoons and silk fibers was established using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. This method was evaluated on the most popu-lar four-way cross hybrid race Kinshu × Showa and its parent races Kinshu and Showa . Most of the peaks observed in the peptide mass fingerprints of the Kinshu × Showa cocoon were from the parent races Kinshu and Showa . Simultaneous acid cleavage of silk fibers at room temperature is a characteristic phenomenon that has not been observed in spider silk and animal hair.
基于基质辅助激光解吸/电离飞行时间质谱的蚕丝生物分型。一、方法开发
建立了一种基质辅助激光解吸/电离飞行时间质谱分析蚕茧和蚕丝纤维的方法。该方法以最流行的四交杂交种“金叔×昭和”及其亲本“金叔”和“昭和”为对象进行了评价。大部分的山峰中观察到的肽质量指纹Kinshu×昭和来自父种族Kinshu和昭和茧。丝纤维在室温下同时发生酸解理是一种在蜘蛛丝和动物毛中尚未观察到的特征现象。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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