Effects of Fenugreek (Trigonella foenumgraecum) Oil On Matrix Metalloprotease type 2 (MMP2) and Fibroblast growth factor type 2 (FGF2) expression during Wounds Healing Process in Diabetic Male Wister Rat

Kufa Journal For Veterinary Medical Sciences Pub Date : 2021-12-30 DOI:10.36326/kjvs/2021/v12i23281

Abeer K.M. Al-Hilaly, H. Al-Salih, Fadhilk Hassooni

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Abstract

Background: Wound healing is a complicated process occurred to reform the integrity and homeostasis of wounded tissue, there is evidence that fenugreek induce and/or enhance the healing process in diabetic in male Wister rats. Aim: To investigate the effects of Fenugreek on levels of matrix metalloprotease type 2 (MMP2) and (FGF2) levels during wound healing process of diabetic male Wister rats via immunohistochemistry techniques. Methods: Thirty diabetic male Wister rats were divided into two main groups each group 15 rats, namely group D )diabetic rats without treatment ( and group FD )diabetic rats treated with 10% fenugreek oil applied locally on wound area(. Five rats were euthanized from each group at week two and week three of experimental period. MMP2 and FGF2 expression levels were measured using immunohistochemistry technique. Results: Fenugreek oil showed the ability to decrease the expression of MMP2 in 10% fenugreek oil treated diabetic rats during week two and week three of wound healing process compared with diabetic rats without treatment. However, Fenugreek oil stimulated high expression of FGF2 in 10% fenugreek oil treated diabetic rats during experimental period compared with diabetic rats without treatment. Conclusion: The results of the present study revealed that fenugreek oil treatment has the ability to reduce the expression of MMP2 and elevate FGF2 expression in 10% fenugreek oil treated diabetic rats compared with non-treated diabetic rats.

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胡芦巴油对糖尿病雄性Wister大鼠伤口愈合过程中基质金属蛋白酶2 (MMP2)和成纤维细胞生长因子2 (FGF2)表达的影响

背景:伤口愈合是一个复杂的过程，是为了改变损伤组织的完整性和稳态而发生的，有证据表明胡芦巴能诱导和/或促进雄性Wister大鼠糖尿病的愈合过程。目的:通过免疫组化技术研究胡芦巴对糖尿病雄性Wister大鼠创面愈合过程中基质金属蛋白酶2 (MMP2)和FGF2水平的影响。方法:将30只雄性糖尿病Wister大鼠分为两组，每组15只，分别为D组(未治疗组)和FD组(创面局部涂10%胡芦巴油治疗组)。实验第2周和第3周，每组取5只大鼠实施安乐死。采用免疫组织化学技术检测MMP2和FGF2的表达水平。结果:与未处理的糖尿病大鼠相比，10%胡芦巴油处理的糖尿病大鼠在伤口愈合过程的第2周和第3周，可以降低MMP2的表达。然而，在实验期间，10%胡芦巴油处理的糖尿病大鼠与未处理的糖尿病大鼠相比，胡芦巴油刺激FGF2的高表达。结论:本研究结果表明，10%胡芦巴油处理的糖尿病大鼠与未处理的糖尿病大鼠相比，具有降低MMP2表达和提高FGF2表达的能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Kufa Journal For Veterinary Medical Sciences

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