Electrochemical acetylcholinesterase biosensor for detection of cholinesterase inhibitors: study with eserine

IF 0.6 4区工程技术 Q4 ENGINEERING, ELECTRICAL & ELECTRONIC

Informacije Midem-Journal of Microelectronics Electronic Components and Materials Pub Date : 2019-02-01 DOI:10.33180/INFMIDEM2018.406

N. Lokar, V. Kononenko, D. Drobne, D. Vrtacnik

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引用次数: 0

Abstract

Cholinesterase inhibitors are widely used as pesticides, as chemical warfare agents and as drugs to treat symptoms of Alzheimer’s disease. Therefore, it is a high need to develop methods for their detection which are fast, sensitive, and reliable. This paper reports a preliminary work in the development of an electrochemical biosensor based on acetylcholinesterase (AChE) which is constructed by immobilization layers – cysteamine/glutaraldehyde/AChE on thin layer gold electrode for detection of cholinesterase inhibitors. Eserine (physostigmine) was used as a test inhibitor. The enzyme immobilization efficacy was evaluated by measuring activity of immobilized enzyme via Ellman’s method. The enzyme activity of the initial reduction of 33% in five days remained after that stable for at least one week. Chronoamperometric response to substrate acetylthiocholine chloride (ATCl) was assumed to follow Michaelis-Menten kinetics. After exposure biosensor to 25 mM eserine for 10 min, 70% inhibition of enzyme was detected. Reactivation factor of inhibited AChE was determined as 0.016 min-1.

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电化学乙酰胆碱酯酶生物传感器检测胆碱酯酶抑制剂的研究

胆碱酯酶抑制剂被广泛用作杀虫剂、化学战剂和治疗阿尔茨海默病症状的药物。因此，迫切需要开发快速、灵敏、可靠的检测方法。本文报道了在薄层金电极上采用半胱胺/戊二醛/乙酰胆碱酯酶(AChE)固定层构建的用于检测胆碱酯酶抑制剂的电化学生物传感器的初步研制工作。鸢尾碱(蛇毒碱)作为试验抑制剂。采用Ellman法测定固定化酶的活性，评价酶的固定化效果。酶活性在最初的5天内降低33%，此后保持稳定至少一周。假设对底物乙酰硫代氯化胆碱(ATCl)的计时电流响应遵循michaelis - menten动力学。将生物传感器暴露于25 mM eserine中10 min后，检测到70%的酶抑制。测定抑制AChE的再激活因子为0.016 min-1。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Informacije Midem-Journal of Microelectronics Electronic Components and Materials 工程技术-材料科学：综合

CiteScore

1.80

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Informacije MIDEM publishes original research papers in the fields of microelectronics, electronic components and materials. Review papers are published upon invitation only. Scientific novelty and potential interest for a wider spectrum of readers is desired. Authors are encouraged to provide as much detail as possible for others to be able to replicate their results. Therefore, there is no page limit, provided that the text is concise and comprehensive, and any data that does not fit within a classical manuscript can be added as supplementary material. Topics of interest include: Microelectronics, Semiconductor devices, Nanotechnology, Electronic circuits and devices, Electronic sensors and actuators, Microelectromechanical systems (MEMS), Medical electronics, Bioelectronics, Power electronics, Embedded system electronics, System control electronics, Signal processing, Microwave and millimetre-wave techniques, Wireless and optical communications, Antenna technology, Optoelectronics, Photovoltaics, Ceramic materials for electronic devices, Thick and thin film materials for electronic devices.