An assessment for in vitro propagation and genetic stability of Phoebe goalparensis Hutchinson, an endemic valuable timber tree of North East India.

IF 2.8 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Journal, genetic engineering & biotechnology Pub Date : 2023-03-10 DOI:10.1186/s43141-023-00487-9

Kalpataru Dutta Mudoi, Barbi Gogoi, Gitasree Borah, Marine Hussain, Tabassum Tasfia, Krishnakhi Borah, Himangshu Lekhak, Siddhartha Proteem Saikia

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Abstract

Background: Phoebe goalparensis is an endemic forest species of North East India that belongs to Lauraceae family. P. goalparensis is used as timbers yielding plants for commercial importance in the local furniture markets of North East India. A rapid in vitro micropropagation protocol was established by using apical and axillary shoot tips on Murashige and Skoog medium with varied concentrations of plant growth regulators.

Results: In this study, 5.0 mg/l BAP augmented medium was chosen as the best for shoot multiplication of the plant. However, IBA (2.0 mg/l) was the most responsive for root induction. Moreover, 70% of root induction was recorded during rooting experiment and 80-85% survivability was observed during the acclimatization of this species. Clonal fidelity of P. goalparensis was determined with ISSR marker and it was observed that in vitro raised plantlets were polymonomorphic.

Conclusion: Hence, an efficient protocol with high proliferation and rooting was established for P. Goalparensis that could aid in massive propagation in future.

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印度东北部特有名木菲比(Phoebe goalparensis Hutchinson)离体繁殖及遗传稳定性评价。

背景:菲比是印度东北部的一种特有森林物种，属于樟科。在印度东北部的当地家具市场，P. goalparensis被用作具有商业重要性的木材生产植物。采用不同浓度的植物生长调节剂，在Murashige和Skoog培养基上建立了芽尖和腋尖离体快速繁殖方案。结果:本研究选择5.0 mg/l BAP增强培养基为植株茎部增殖的最佳培养基。而IBA (2.0 mg/l)对根诱导的响应最大。在生根试验中记录了70%的生根诱导率，在驯化过程中观察到80-85%的成活率。用ISSR标记法测定了金凤花的克隆保真度，结果表明，金凤花体外培养植株为多单态植株。结论:建立了一种高效、高增殖、高生根的培养方法，为今后的大规模繁殖奠定了基础。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Journal, genetic engineering & biotechnology

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