EDTA plasma in glass and polyethylene terephthalate tubes is suitable for measurement of B-type natriuretic peptide on the Mindray CL-6000i.

IF 2.1 4区医学 Q3 MEDICAL LABORATORY TECHNOLOGY

Annals of Clinical Biochemistry Pub Date : 2023-09-01 Epub Date: 2023-03-14 DOI:10.1177/00045632231162289

Wei Zhao, Gang Feng, Jun Wan, Xing Wang, Jiping Feng, Wanwan Zhang, Yalan Yu, Rui Pu, Yong Du, Yongtong Cao

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引用次数: 0

Abstract

Aims: To explore differences in B-type natriuretic peptide (BNP) concentration and stability and evaluate BNP accuracy in different collection tubes.

Methods: BNP concentrations in heparin/glass, EDTA/glass, and EDTA/polyethylene terephthalate (PET) tubes were measured on the Mindray CL-6000i at 0.5, 1, 2, and 4 h after collection. Differences were evaluated using Wilcoxon's paired tests and Bland-Altman plots. BNP stability and measurement accuracies were estimated using Kruskal-Wallis H tests and recovery tests.

Results: BNP concentrations in EDTA/glass tubes were 31.4% higher than those in heparin/glass tubes and 3.04% lower than those in EDTA/PET tubes. BNP stability significantly decreased in the heparin/glass tube. BNP remained stable in EDTA/glass and EDTA/PET tubes at room temperature for 4 h. BNP recovery rates in heparin/glass, EDTA/glass, and EDTA/PET tubes were 77.46, 86.04, and 88.23%, respectively.

Conclusions: Plasma in EDTA/glass and EDTA/PET tubes is suitable for BNP measurement on the Mindray CL-6000i.

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玻璃管和聚对苯二甲酸乙二醇酯管中的EDTA血浆适用于在迈瑞CL-6000i上测量B型钠尿肽。

目的：探讨不同收集管中B型钠尿肽（BNP）浓度和稳定性的差异，并评估BNP的准确性。方法：在收集后0.5、1、2和4小时，在迈瑞CL-6000i上测量肝素/玻璃、EDTA/玻璃和EDTA/聚对苯二甲酸乙二醇酯（PET）管中的BNP浓度。使用Wilcoxon配对检验和Bland-Altman图评估差异。使用Kruskal-Wallis H试验和回收率试验估计BNP的稳定性和测量精度。结果：EDTA／玻璃管中BNP浓度比肝素／玻璃管高31.4%，比EDTA／PET管低3.04%。肝素/玻璃管中的BNP稳定性显著降低。BNP在室温下在EDTA/玻璃和EDTA/PET管中保持稳定4h。肝素/玻璃管、EDTA/玻璃管和EDTA/PET管中的BNP回收率分别为77.46%、86.04和88.23%。结论：EDTA/玻璃管和EDTA/PET管中的血浆适合于在迈瑞CL-6000i上测量BNP。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Annals of Clinical Biochemistry Biochemistry, Genetics and Molecular Biology-Clinical Biochemistry

CiteScore

5.20

自引率

4.50%

发文量

期刊介绍： Annals of Clinical Biochemistry is the fully peer reviewed international journal of the Association for Clinical Biochemistry and Laboratory Medicine. Annals of Clinical Biochemistry accepts papers that contribute to knowledge in all fields of laboratory medicine, especially those pertaining to the understanding, diagnosis and treatment of human disease. It publishes papers on clinical biochemistry, clinical audit, metabolic medicine, immunology, genetics, biotechnology, haematology, microbiology, computing and management where they have both biochemical and clinical relevance. Papers describing evaluation or implementation of commercial reagent kits or the performance of new analysers require substantial original information. Unless of exceptional interest and novelty, studies dealing with the redox status in various diseases are not generally considered within the journal''s scope. Studies documenting the association of single nucleotide polymorphisms (SNPs) with particular phenotypes will not normally be considered, given the greater strength of genome wide association studies (GWAS). Research undertaken in non-human animals will not be considered for publication in the Annals. Annals of Clinical Biochemistry is also the official journal of NVKC (de Nederlandse Vereniging voor Klinische Chemie) and JSCC (Japan Society of Clinical Chemistry).