The Simple Method for Immunohistochemistry (IHC) Analyzing of Intensity using Corel Photo-Paint® with CMYK Split-channel

Abstract

Immunohistochemistry (IHC) is a popular method to detect protein in the tissue. But, determining protein level in tissue is using ELISA which needs more cost. Although, the intensity of IHC color relates to an amount in tissue. Therefore, image processing to measure the intensity of IHC quantitively was developed using much software. The common software used in Indonesia is Corel Photo-Paint® (CCP). This article described the simple method to measure the IHC intensity using CCP based on Cyan-Magenta-YellowBlack (CMYK) Split channel. The conclusions were CCP could be used for image processing software to measure the IHC intensity. The higher intensity has fewer grey level, the lower intensity has a higher grey level