Determinations of the effects of cyfluthrin on the hemocytes parameters of freshwater mussel (Unio delicatus)

Abstract

The overuse of pesticides has been increasing since the 20th century. Depending on this use, non-target organisms are also affected apart from target organisms. Cyfluthrin is a synthetic pyrethroid pesticide used in agriculture, domestic and veterinary medicine against insects. It may also affect non-target aquatic organisms as a result of mixing with aquatic ecosystems. This study was aimed to investigate the effect of cyfluthrin on freshwater mussels, one of the aquatic invertebrate species, with hemocyte parameters. Total hemolymph counts, hemolymph cell morphology, and differential hemocyte counts were performed from hemolymph taken from mussels exposed to different cyfluthrin doses exposures for 24 and 48 hours. Compared to the control group, the total hemocyte counts of the experimental groups were found to increase in 24h and decrease in 48h significantly (p<0.05). In the examination of hemocyte morphologies, granular, semi granular, and hyalinocyte cells were observed. Similar values of differential hemocyte counts were found both 24 and 48h exposure times. As a result, in aquatic toxicology studies, besides total hemocyte count, analysis of hemocyte morphologies and differential hemocyte counts are found to be good biomarkers.