Integrative approach for discovering of the new species within the genus Allocreadium Looss, 1900 (Trematoda: Allocreadiidae) and framing of biogeographical hypotheses for the genus.

IF 1 4区医学 Q4 PARASITOLOGY

Systematic Parasitology Pub Date : 2023-04-01 DOI:10.1007/s11230-022-10081-1

Konstantin S Vainutis, Anastasia N Voronova, Misako Urabe, Vitaly M Kazarin

{"title":"Integrative approach for discovering of the new species within the genus Allocreadium Looss, 1900 (Trematoda: Allocreadiidae) and framing of biogeographical hypotheses for the genus.","authors":"Konstantin S Vainutis, Anastasia N Voronova, Misako Urabe, Vitaly M Kazarin","doi":"10.1007/s11230-022-10081-1","DOIUrl":null,"url":null,"abstract":"<p><p>In July 2012 new Allocreadium species was isolated from Carassius gibelio caught in the Arsenyevka River, Primorsky region, Russia. Analyses on the morphometrics and internal organs' topology revealed that these worms are morphologically closest with A. isoporum but both species are independent according to high genetic distances based on the 28S gene fragment (5.434±0.0073%). Unlike A. isoporum found earlier in Europe, the new species named A. pseudoisoporum sp. nov. has a shorter body length and the vitellarium not reaching the posterior end of the body at some distance and its anterior border is on the level of the ventral sucker. Allocreadium pseudoisoporum sp. nov. differs from seven species previously found in the Russian Far East with the following features: smaller size of the body, suckers' ratio, range values, and topology of internal structures. Newly localities in the Pavlovka River and the Artyomovka River were discovered for A. khankaiensis. Morphological variability of the worms from the Pavlovka River was observed in comparison with A. khankaiensis from the Komissarovka River. Using scanning electron microscope, we examined external surfaces of three species (A. pseudoisoporum sp. nov., A. khankaiensis, A. hemibarbi) and observed structures reminiscent sensory receptors. This study was aimed to describe species diversity of allocreadiids inhabiting the south of Primorsky region, and to clarify phylogenetic relationships between the species from the genus Allocreadium Looss, 1900 using molecular genetic methods. The phylogenetic Bayesian tree based on the 28S gene showed a clear separation of ten Allocreadium species and confirmed the validity of A. pseudoisoporum sp. nov. Allocreadium pseudoisoporum sp. nov. is most similar to A. gotoi (genetic distances - 3.578±0.0051% in 28S, and 18.777±0.0149% in cox1), and represented the earliest divergent lineage in Allocreadium clade on the phylogenetic tree based on the 28S gene, thereby indicating its proximity to the ancestral node. Also, dichotomous keys for 25 Palearctic species of Allocreadium were prepared based on the morphology of the adult worms.</p>","PeriodicalId":54436,"journal":{"name":"Systematic Parasitology","volume":null,"pages":null},"PeriodicalIF":1.0000,"publicationDate":"2023-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"4","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Systematic Parasitology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1007/s11230-022-10081-1","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"PARASITOLOGY","Score":null,"Total":0}

引用次数: 4

Abstract

In July 2012 new Allocreadium species was isolated from Carassius gibelio caught in the Arsenyevka River, Primorsky region, Russia. Analyses on the morphometrics and internal organs' topology revealed that these worms are morphologically closest with A. isoporum but both species are independent according to high genetic distances based on the 28S gene fragment (5.434±0.0073%). Unlike A. isoporum found earlier in Europe, the new species named A. pseudoisoporum sp. nov. has a shorter body length and the vitellarium not reaching the posterior end of the body at some distance and its anterior border is on the level of the ventral sucker. Allocreadium pseudoisoporum sp. nov. differs from seven species previously found in the Russian Far East with the following features: smaller size of the body, suckers' ratio, range values, and topology of internal structures. Newly localities in the Pavlovka River and the Artyomovka River were discovered for A. khankaiensis. Morphological variability of the worms from the Pavlovka River was observed in comparison with A. khankaiensis from the Komissarovka River. Using scanning electron microscope, we examined external surfaces of three species (A. pseudoisoporum sp. nov., A. khankaiensis, A. hemibarbi) and observed structures reminiscent sensory receptors. This study was aimed to describe species diversity of allocreadiids inhabiting the south of Primorsky region, and to clarify phylogenetic relationships between the species from the genus Allocreadium Looss, 1900 using molecular genetic methods. The phylogenetic Bayesian tree based on the 28S gene showed a clear separation of ten Allocreadium species and confirmed the validity of A. pseudoisoporum sp. nov. Allocreadium pseudoisoporum sp. nov. is most similar to A. gotoi (genetic distances - 3.578±0.0051% in 28S, and 18.777±0.0149% in cox1), and represented the earliest divergent lineage in Allocreadium clade on the phylogenetic tree based on the 28S gene, thereby indicating its proximity to the ancestral node. Also, dichotomous keys for 25 Palearctic species of Allocreadium were prepared based on the morphology of the adult worms.

Abstract Image

查看原文本刊更多论文

异藻属新种的综合发现方法及该属生物地理学假说的构建。

2012年7月，在俄罗斯滨海边疆区Arsenyevka河捕获的gibelio Carassius中分离到异藻新种。形态计量学和内脏拓扑学分析表明，这两种虫在形态上与异孔虫最接近，但基于28S基因片段的高遗传距离(5.434±0.0073%)表明，这两种虫是独立的。与早前在欧洲发现的异孔拟虫不同，新物种伪异孔拟虫体长较短，喙部距身体后端有一定距离，其前缘与腹侧吸盘齐平。异creadium pseudoisoporum sp. 11 .不同于先前在俄罗斯远东地区发现的七个物种，具有以下特征:较小的身体尺寸，吸盘比例，范围值和内部结构的拓扑结构。在Pavlovka河和Artyomovka河的新地点发现了A. khankaiensis。对来自帕夫洛夫卡河和科米萨罗夫卡河的A. khankaiensis的形态差异进行了比较。利用扫描电子显微镜观察了三种植物(A. pseudoisoporum sp. nov, A. khankaiensis, A. hemibarbi)的外表面结构，并观察了它们的感受器结构。本研究旨在用分子遗传学方法描述生活在滨海边疆区南部的allocreadiids的物种多样性，并阐明Allocreadium Looss, 1900属物种间的系统发育关系。基于28S基因的系统发育贝叶斯树显示了10个异种的明显分离，证实了a . pseudoisoporum sp. 11 .与a . gotoi最相似(遗传距离为- 3.578±0.0051%，cox1遗传距离为18.777±0.0149%)，在基于28S基因的系统发育树上代表了异源亚种中最早的分化谱系，从而表明其与祖先节点的接近性。此外，还根据成虫的形态特征，编制了25种古北异虫的二分类键。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Systematic Parasitology 医学-寄生虫学

CiteScore

2.20

自引率

23.10%

发文量

审稿时长

6-12 weeks

期刊介绍： Systematic Parasitology publishes papers on the systematics, taxonomy and nomenclature of the following groups: Nematoda (including plant-parasitic), Monogenea, Digenea, Cestoda, Acanthocephala, Aspidogastrea, Cestodaria, Arthropoda (parasitic copepods, hymenopterans, mites, ticks, etc.), Protozoa (parasitic groups), and parasitic genera in other groups, such as Mollusca, Turbelleria, etc. Systematic Parasitology publishes fully illustrated research papers, brief communications, and fully illustrated major revisions. In order to maintain high standards, all contributors describing new taxa are asked to state clearly where the holotype is deposited and to make paratypes available for examination by the referees. It is recognized that, in some cases, this may cause problems for the authors, but it is hoped that by adhering to this rule authors may be protected against rapid synonymy of their taxa, and the types will be preserved for posterity.