LYMPHOCYTE ENZYMES AS AN INDICATOR OF IMMUNE RESPONSE ACTIVITY IN IXODID TICK-BORNE BORRELIOSIS

M. Avdeeva, D. Moshkova, Lyudmila P. Blazhnyaya, Yelena V. Kozyreva
{"title":"LYMPHOCYTE ENZYMES AS AN INDICATOR OF IMMUNE RESPONSE ACTIVITY IN IXODID TICK-BORNE BORRELIOSIS","authors":"M. Avdeeva, D. Moshkova, Lyudmila P. Blazhnyaya, Yelena V. Kozyreva","doi":"10.17816/eid121351","DOIUrl":null,"url":null,"abstract":"Aim of the study was to determine the pathogenetic mechanisms of the infectious and inflammatory process in the erythemal form of acute ITB based on the study of the activity of lysosomal enzymes of lymphocytes, the level of IL-2 and the clinical manifestations of the disease. \nMethods: The main group is represented by 609 patients hospitalized at the Krasnodar City Clinical Hospital for the period 20102019. The study group consisted of 45 patients with erythemal form of acute ITB. In the dynamics of the disease, the level of IL-2 and the cytochemical activity of acid phosphatase (AP) and nonspecific alpha-naphthyl esterase (-NAE) of lymphocytes were determined. \nResults: during the height of ITB, a decrease in the cytochemical activity of hydrolytic enzymes of lymphocytes was noted in comparison with the control group. IL-2 levels had a high dispersion and were associated with the clinical manifestations of the disease. A low level of IL-2 correlated with a decrease in the activity of -NAE lymphocytes. During the period of convalescence, there was a restoration of the enzymatic activity of lymphocytes, an increase in the number of cells with a pronounced activity of -NAE, typical of T-lymphocytes with killer activity. \nConclusion: The hydrolytic enzymes of the lysosomes of CP and -NAE lymphocytes make it possible to judge the intensity of intracellular metabolic processes and, in combination with the clinical symptoms of the disease and the activity of IL-2, are an indicator of the state of the immune process, supplementing the results of traditional immunological studies in patients with the erythema form of acute ixodid tick-borne borreliosis. The predominance of enzymatically inactive forms of T-lymphocytes in the acute period of the disease reflects a certain deficiency of the T-cell immunity.","PeriodicalId":93465,"journal":{"name":"Journal of infectious diseases and epidemiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2023-02-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of infectious diseases and epidemiology","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.17816/eid121351","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0

Abstract

Aim of the study was to determine the pathogenetic mechanisms of the infectious and inflammatory process in the erythemal form of acute ITB based on the study of the activity of lysosomal enzymes of lymphocytes, the level of IL-2 and the clinical manifestations of the disease. Methods: The main group is represented by 609 patients hospitalized at the Krasnodar City Clinical Hospital for the period 20102019. The study group consisted of 45 patients with erythemal form of acute ITB. In the dynamics of the disease, the level of IL-2 and the cytochemical activity of acid phosphatase (AP) and nonspecific alpha-naphthyl esterase (-NAE) of lymphocytes were determined. Results: during the height of ITB, a decrease in the cytochemical activity of hydrolytic enzymes of lymphocytes was noted in comparison with the control group. IL-2 levels had a high dispersion and were associated with the clinical manifestations of the disease. A low level of IL-2 correlated with a decrease in the activity of -NAE lymphocytes. During the period of convalescence, there was a restoration of the enzymatic activity of lymphocytes, an increase in the number of cells with a pronounced activity of -NAE, typical of T-lymphocytes with killer activity. Conclusion: The hydrolytic enzymes of the lysosomes of CP and -NAE lymphocytes make it possible to judge the intensity of intracellular metabolic processes and, in combination with the clinical symptoms of the disease and the activity of IL-2, are an indicator of the state of the immune process, supplementing the results of traditional immunological studies in patients with the erythema form of acute ixodid tick-borne borreliosis. The predominance of enzymatically inactive forms of T-lymphocytes in the acute period of the disease reflects a certain deficiency of the T-cell immunity.
淋巴细胞酶作为蜱传疏螺旋体病免疫反应活性的指标
本研究旨在通过对淋巴细胞溶酶体酶活性、白细胞介素-2水平及该病临床表现的研究,探讨急性ITB红斑型感染及炎症过程的发病机制。方法:以2010 - 2019年在克拉斯诺达尔市临床医院住院的609例患者为主要研究对象。研究组由45例红斑型急性ITB患者组成。在疾病动态中,测定白细胞介素2水平和淋巴细胞酸性磷酸酶(AP)和非特异性α -萘酯酶(-NAE)的细胞化学活性。结果:与对照组相比,在ITB高峰期,淋巴细胞水解酶的细胞化学活性降低。IL-2水平具有高弥散性,并与疾病的临床表现相关。低水平的IL-2与-NAE淋巴细胞活性降低相关。恢复期淋巴细胞酶活性恢复,具有明显-NAE活性的细胞数量增加,具有杀伤活性的典型t淋巴细胞。结论:CP和-NAE淋巴细胞溶酶体的水解酶可以判断细胞内代谢过程的强度,并结合疾病的临床症状和IL-2的活性,是免疫过程状态的一个指标,补充了传统的免疫学研究结果。在疾病的急性期,酶失活形式的t淋巴细胞的优势反映了t细胞免疫的一定缺陷。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
自引率
0.00%
发文量
0
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信