Effect of extremely low frequency magnetic field in growth, CMCase, electric conductivity and DNA of Aspergillus niger

The Journal of Applied Sciences Research Pub Date : 2020-03-23 DOI:10.21608/ejphysics.2020.29413.1042

Sahar E. Abo-Neima, M. El-Metwally

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Abstract

In the past years the extremely low frequency magnetic field (ELF-MF) of the millimeter range has been actively used in biology, medicine, biotechnology, in this study the impact of electromagnetic radiations in growth, carboxymethycellulase activity (CMCase), cell conductivity and RAPD DNA pattern of A. niger were investigated. Experimentally, when the fungus treated with magnetic field at constant intensity 50Hz, 10 mT at different exposure times for five days (2 h/day) the total biomass dry weight inhibited by 72.2% after the first 2 h. ELF-MF showed also inhibition effect of in CMCase activity of A. niger. The enzyme activity was decreased from 30 IU/ml to 22.5 IU/ml after only 2h of exposure and the treated fungus lost about 50% of its CMCase activity after 10 h.The treated fungus showed also decreased in conductivity of fungal cells from 1.78×10 -1 to 0.51×10 -5 Siemens/meter. The study extended to detect the changes in RAPD patterns of A. niger DNA before and after exposure to the 50 Hz frequency. In conclusion, ELF-MF can be used as a safe method in control the activity of higly resistant fungi as A. niger .

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极低频磁场对黑曲霉生长、CMCase、电导率和DNA的影响

近年来，毫米范围的极低频磁场(ELF-MF)在生物学、医学、生物技术等领域得到了广泛的应用，本文研究了电磁辐射对黑曲霉生长、羧甲基纤维素酶活性(CMCase)、细胞电导率和RAPD DNA图谱的影响。实验结果表明，在恒定强度50Hz、10 mT、不同暴露次数的磁场条件下处理5 d (2 h/d)，前2 h后总生物量干重下降72.2%，ELF-MF对黑僵菌的CMCase活性也有抑制作用。暴露2h后，酶活性从30 IU/ml下降到22.5 IU/ml, 10 h后，真菌的CMCase活性下降了约50%，真菌细胞的电导率也从1.78×10 -1下降到0.51×10 -5西门子/米。该研究扩展到检测暴露于50 Hz频率前后黑曲霉DNA RAPD模式的变化。综上所述，ELF-MF可以作为一种安全的方法来控制黑曲霉等高抗性真菌的活性。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The Journal of Applied Sciences Research

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