Isolation and Characterization of Endofytic Bacteria Indigenus Potentially Producing IAA (Indole Acetic Acid) in West Sumatera and Their Effect on Nursery Palm Oil (Elaeis guineensis jacq)

Rover Rover, R. Mayerni, Y. Yanti, A. Syarif
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Abstract

The IAA potentials of endophytic bacteria indigenous isolated from roots palm oil in west Sumatra. Isolate were characterized on the basis of visual observation, gram staining, hypersensitive reaction and IAA producing bacteria.  Subsequently, effect on plant growth was tested by nursery palm oil used random design block. Out of 82 isolates, base on gram test 12 isolates were gram negative and 70 isolates grams positive and then results on the reaction hypersensitive test (HR test) there are 8 isolates that positive which means the isolate cannot be applied on the soil and plant. Eighty isolates selected were able to produce IAA that was between ppm 0,30ppm - 3,65ppm. Seven isolates showed clearly the growth promoting plant under field condition. Hence, these isolates are promising plant growth promoting isolates showing multiple attributes that can significantly influence the nursery palm oil. The result of present study, treatment E3.1.2 has higher plant most of the other treatment i.e. 21,93 cm and number of leaves i.e. 4,33 leaves.
西苏门答腊产吲哚乙酸内生菌的分离、鉴定及其对苗木棕榈油的影响
西苏门答腊岛根棕榈油内生细菌的IAA电位研究。通过目视观察、革兰氏染色、过敏反应和产生IAA的细菌对分离物进行了鉴定。随后,采用随机设计块,检测苗圃棕榈油对植物生长的影响。在82株分离株中,革兰氏试验结果为革兰氏阴性12株,阳性70株,反应过敏试验结果为阳性8株,不能用于土壤和植物。所选的80株菌株能够产生ppm在0,30ppm - 3,65ppm之间的IAA。7个分离株在田间条件下表现出明显的促生作用。因此,这些分离株是有希望的植物生长促进分离株,显示出可以显著影响苗圃棕榈油的多种属性。本研究结果表明,处理E3.1.2植株最高,为21,93 cm,叶片数为4,33片。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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