Partial characterization of digestive proteases in Totoaba (Totoaba macdonaldi)

IF 0.9 4区生物学 Q3 MARINE & FRESHWATER BIOLOGY

Marine and Freshwater Behaviour and Physiology Pub Date : 2019-07-04 DOI:10.1080/10236244.2019.1662724

Mabelyn Córdova-Montejo, Miguel Sáenz De Rodrigáñez García, Emyr Saul Peña-Marín, R. Martínez‐García, S. Camarillo‐Coop, L. López, M. Galaviz, C. Álvarez‐González

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引用次数: 1

Abstract

ABSTRACT The objective of this study was to characterize the digestive proteases of totoaba (Totoaba macdonaldi). Fish were sacrificed to obtain the multienzymatic extracts from the stomach and intestine, and determine the stability and optimum pH and temperature values. Residual activity and number of isoforms were determined with some inhibitors. Optimal pH of stomach proteases was 2, with stability above 100% at that same pH. Optimum pH of intestinal proteases was between 9 and 11, with stability above 100% between 8-12. Optimum temperature for stomach proteases was 35°C and remained highly stable, while optimum temperature for intestinal proteases was 45°C, with high stability between 35-55°C. Pepstatin A totally inhibited acid protease activity and revealed a single band. SDS-PAGE zymogram revealed 8 bands in the intestine, where phenanthroline inhibited 80% of the total activity. The digestive capacity of T. macdonaldi is characteristic of a strict carnivore, similar to other marine fish species.

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石首鱼(Totoaba macdonaldi)消化性蛋白酶的部分鉴定

摘要本研究的目的是表征石首鱼(totoaba macdonaldi)的消化蛋白酶。通过牺牲鱼的胃和肠来获得多酶提取物，并确定其稳定性和最佳pH值和温度。用一些抑制剂测定了残留活性和同工异构体的数量。胃蛋白酶的最适pH为2，在相同pH下稳定性在100%以上;肠蛋白酶的最适pH为9 ~ 11，在8 ~ 12之间稳定性在100%以上。胃蛋白酶的最适温度为35℃，且保持高度稳定;肠蛋白酶的最适温度为45℃，在35-55℃之间具有较高的稳定性。Pepstatin A完全抑制酸性蛋白酶活性，呈单条带。SDS-PAGE酶谱显示肠内有8个条带，其中菲罗啉抑制了80%的总活性。T. macdonaldi的消化能力是一种严格的食肉动物的特征，类似于其他海洋鱼类。

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来源期刊

Marine and Freshwater Behaviour and Physiology 生物-海洋与淡水生物学

CiteScore

2.10

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Marine and Freshwater Behaviour and Physiology is devoted to the publication of papers covering field and laboratory research into all aspects of the behaviour and physiology of all marine and freshwater animals within the contexts of ecology, evolution and conservation. As the living resources of the world’s oceans, rivers and lakes are attracting increasing attention as food sources for humans and for their role in global ecology, the journal will also publish the results of research in the areas of fisheries biology and technology where the behaviour and physiology described have clear links to the contexts mentioned above. The journal will accept for publication Research Articles, Reviews, Rapid Communications and Technical Notes (see Instructions for authors for details). In addition, Editorials, Opinions and Book Reviews (invited and suggested) will also occasionally be published. Suggestions to the Editor-In-Chief for Special Issues are encouraged and will be considered on an ad hoc basis. With the goal of supporting early career researchers, the journal particularly invites submissions from graduate students and post-doctoral researchers. In addition to recognising the time constraints and logistical limitations their research often faces, and their particular need for a prompt review process, accepted articles by such researchers will be given prominence within the journal (see Instructions for authors for details).