Salt tolerance's toll: prolonged exposure to saline water inflicts damage to the blood cells of dice snakes ( Natrixtessellata )

IF 2.3 3区 环境科学与生态学 Q2 ECOLOGY
Web Ecology Pub Date : 2017-01-24 DOI:10.5194/WE-17-1-2017
Vanya Koleva, Yurii V. Kornilev, Ivan Telenchev, Simeon Lukanov, B. Hristova, N. Natchev
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引用次数: 8

Abstract

Abstract. Dice snakes (Natrix tessellata, Laurenti, 1768) inhabit oligohaline and brackish waters along the Bulgarian Black Sea coast, where they often forage at sea. Under these conditions, this species should tolerate highly variable blood plasma sodium concentrations for extended time periods, but the effect of high sodium concentrations to blood cell morphology and physiology is largely unknown. In this experiment, we placed adult dice snakes in waters with different salinity for 84 h, representing the three typical saline concentrations in which dice snakes live and forage: 0.2 ‰ NaCl, fresh water, 16 ‰ NaCl, Black Sea water and 36 ‰ NaCl, World Ocean water. We collected blood through cardiocentesis after exposure to each treatment and used Giemsa's solution for staining the formed elements. We registered numerous nuclear abnormalities (nuclear buds, lobes and blebs) in the blood cells of all investigated specimens. In the snakes placed in the waters with high saline concentration (16 and 36 ‰ NaCl) we found numerous cells with ruptured membranes. These snakes also showed increased mitochondrial activity compared to those in fresh water (1.85 and 2.53 times higher, respectively). Our data suggest that even though dice snakes show a remarkable tolerance to waters with increased salinity, prolonged exposure to it induces significant physiological stress in N. tessellata, which poses a clear limit for their ability to stay in the sea for a long time.
耐盐的代价:长时间暴露在盐水中会对骰子蛇的血细胞造成损害(Natrixtessellata)
摘要骰子蛇(Natrix tessellata, Laurenti, 1768)栖息在保加利亚黑海沿岸的低盐和半咸淡水中,它们经常在海上觅食。在这些条件下,该物种应该能够长时间耐受高度变化的血浆钠浓度,但高钠浓度对血细胞形态和生理的影响在很大程度上是未知的。在本实验中,我们将成年骰子蛇放置在不同盐度的水中84 h,代表了骰子蛇生活和觅食的三种典型盐度浓度:0.2 ‰NaCl,淡水,16 ‰NaCl,黑海和36 ‰NaCl,世界海水。暴露于每种治疗后,我们通过心脏穿刺收集血液,并使用吉姆萨溶液对形成的元素进行染色。我们在所有研究标本的血细胞中记录了许多核异常(核芽、核叶和核泡)。在高盐浓度(16和36 ‰NaCl)的水中,我们发现了许多细胞膜破裂的细胞。与淡水中的蛇相比,这些蛇的线粒体活性也有所增加(分别高出1.85倍和2.53倍)。我们的数据表明,尽管骰子蛇对盐度增加的水表现出显著的耐受性,但长时间暴露在盐度增加的水中会对N. tessellata产生显著的生理压力,这明显限制了它们在海洋中长时间停留的能力。
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来源期刊
Web Ecology
Web Ecology Agricultural and Biological Sciences-Ecology, Evolution, Behavior and Systematics
CiteScore
4.60
自引率
0.00%
发文量
6
审稿时长
17 weeks
期刊介绍: Web Ecology (WE) is an open-access journal issued by the European Ecological Federation (EEF) representing the ecological societies within Europe and associated members. Its special value is to serve as a publication forum for national ecological societies that do not maintain their own society journal. Web Ecology publishes papers from all fields of ecology without any geographic restriction. It is a forum to communicate results of experimental, theoretical, and descriptive studies of general interest to an international audience. Original contributions, short communications, and reviews on ecological research on all kinds of organisms and ecosystems are welcome as well as papers that express emerging ideas and concepts with a sound scientific background.
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