Cytotoxic effect of paraprobiotic yogurts on cancer cell lines

Mitra Molaee Parvarei, A. Mortazavian
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Abstract

Cancer is a cellular disorder caused by changes in the mechanisms that control cell growth and differentiation. Breast cancer is the second most common cancer in women following lung cancer. Today, due to the side effects of treatment methods, many efforts have been made to discover natural compounds with a selective power in limiting cancers. This study was performed to investigate the cytotoxicity effects of paraprobiotic yogurt on breast and rectal cancer cell lines (MDA-Md, SKBR3, and SW 480). After culturing and amplifying the cancer cell line to determine the effect of supernatant toxicity, these cells were exposed to different doses of paraprobiotic supernatant, which was performed after 72 hours of MTT assay. According to the results, after adding different dilutions of paraprobiotic yogurt supernatant, YB-B could significantly inhibit the cell viability in three categories of cancer cells (MDA-Md, SKBR3, and SW 480) and had the greatest impact on SKBR3 (p≤0.05). The effects of cytotoxicity increased with elevating the concentration of the extract with the highest percentage of growth inhibition being related to the concentrations of 1/4, 1/3, and 1/2 (p = 0.05 and p = 0.01), respectively. Thus, the use of paraprobiotic yogurt supernatant as an effective substance in the treatment of cancer is recommended. Accordingly, future research could explore its substances to be potentially used in the treatment of cancer.
副益生菌酸奶对癌细胞的细胞毒作用
癌症是一种由控制细胞生长和分化的机制变化引起的细胞疾病。乳腺癌是女性中仅次于肺癌的第二大常见癌症。今天,由于治疗方法的副作用,人们已经做出了许多努力,以发现具有选择性限制癌症的天然化合物。本研究旨在研究益生菌酸奶对乳腺癌和直肠癌细胞系(MDA-Md、SKBR3和SW 480)的细胞毒性作用。癌细胞培养扩增后,将细胞暴露于不同剂量的副益生菌上清液中,进行72小时的MTT实验。结果显示,添加不同稀释度的副益生菌酸奶上清液后,YB-B均能显著抑制3类癌细胞(MDA-Md、SKBR3和SW 480)的细胞活力,其中对SKBR3的影响最大(p≤0.05)。细胞毒作用随提取物浓度的升高而增强,其中生长抑制率最高的浓度分别为1/4、1/3和1/2 (p = 0.05和p = 0.01)。因此,推荐使用益生菌酸奶上清液作为治疗癌症的有效物质。因此,未来的研究可以探索其物质,以潜在地用于治疗癌症。
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