Cytotoxic effect of paraprobiotic yogurts on cancer cell lines

Abstract

Cancer is a cellular disorder caused by changes in the mechanisms that control cell growth and differentiation. Breast cancer is the second most common cancer in women following lung cancer. Today, due to the side effects of treatment methods, many efforts have been made to discover natural compounds with a selective power in limiting cancers. This study was performed to investigate the cytotoxicity effects of paraprobiotic yogurt on breast and rectal cancer cell lines (MDA-Md, SKBR3, and SW 480). After culturing and amplifying the cancer cell line to determine the effect of supernatant toxicity, these cells were exposed to different doses of paraprobiotic supernatant, which was performed after 72 hours of MTT assay. According to the results, after adding different dilutions of paraprobiotic yogurt supernatant, YB-B could significantly inhibit the cell viability in three categories of cancer cells (MDA-Md, SKBR3, and SW 480) and had the greatest impact on SKBR3 (p≤0.05). The effects of cytotoxicity increased with elevating the concentration of the extract with the highest percentage of growth inhibition being related to the concentrations of 1/4, 1/3, and 1/2 (p = 0.05 and p = 0.01), respectively. Thus, the use of paraprobiotic yogurt supernatant as an effective substance in the treatment of cancer is recommended. Accordingly, future research could explore its substances to be potentially used in the treatment of cancer.