Effect of N-acetylcysteine on γ-radiation-induced cytotoxicity in human hepatocellular carcinoma cells

Biomedicine & Aging Pathology Pub Date : 2014-10-01 DOI:10.1016/j.biomag.2014.07.010

Heba H. Mansour , Samia A. Shouman

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引用次数: 1

Abstract

This study investigated the effect of N-acetylcysteine (NAC) against γ-radiation-induced cytotoxicity in human hepatocellular carcinoma (HepG2) cells. HepG2 Cells were incubated with 20 mM of NAC for 24 h prior to 6 Gy γ-irradiation. Apoptosis markers, such as caspase-3 and DNA fragmentation and oxidative stress markers, such as total nitrate/nitrite (NO(x)) and malondialdehyde (MDA) levels, superoxide dismutase (SOD) and glutathione (GSH) content were studied. Half of the lethal dose (LD₅₀) of NAC on HepG2 cell viability was found to be 20 mM/mL after incubation for 48 h. Incubation of irradiated HepG2 cells with NAC inhibited γ-radiation-induced increases in caspase-3 activity and DNA fragmentation. Treatment with NAC before γ-radiation restored the changes induced by γ-irradiation by increasing SOD activity and GSH content in parallel with a decrease in MDA and NO(x) levels in HepG2 cells. NAC has a modulatory effect against γ-radiation-induced oxidative damage plausibly ascribable to its antioxidant/free radical scavenging ability.

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n-乙酰半胱氨酸对γ辐射诱导的人肝癌细胞毒性的影响

本研究探讨了n-乙酰半胱氨酸(NAC)对γ辐射诱导的人肝癌(HepG2)细胞毒性的影响。6 Gy γ辐照前，用20 mM NAC孵育HepG2细胞24 h。研究凋亡标志物，如caspase-3和DNA断裂，氧化应激标志物，如总硝酸盐/亚硝酸盐(NO(x))和丙二醛(MDA)水平，超氧化物歧化酶(SOD)和谷胱甘肽(GSH)含量。NAC对HepG2细胞活性的半数致死剂量(LD50)为20 mM/mL。NAC可抑制γ辐射诱导的HepG2细胞caspase-3活性和DNA片段化的增加。NAC在γ辐照前处理HepG2细胞，通过提高SOD活性和GSH含量，同时降低MDA和NO(x)水平，恢复了γ辐照引起的变化。NAC对γ辐射引起的氧化损伤具有调节作用，这可能归因于其抗氧化/自由基清除能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Biomedicine & Aging Pathology

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