A novel approach to analyze total amino acids contents of food samples by computational image scanning densitometry

Abstract

In the current work, computational image scanning densitometry technique was used instead of conventional and traditional instruments like IC, HPLC, GC and UV/visible spectroscopy for quantification of micro samples of amino acids (lysine hydrochloride, β-alanine, asparagine monohydrate, glutamic acid). The general reaction between locating reagent and amino acid is: This technique has accuracy at the parts per billion level and precision as indicated from percent recovery from sample analysis, which is quite good. Due to certain limits associated with conventional techniques, this technique may be presented as a progressive and important event in the history of evaluation at micro level. Keywords: amino acids, densitometry, micro level.