NEMATICIDAL AND NEMATOSTATIC EFFICACY OF VEGETABLE JUICES OF SOME REPRESENTATIVES OF THE FAMILY SOLANACEAE

Abstract

The effect of the tested substances on in vitro nematicidity and nematostaticity was verified on saprobiotic (Caenorhabditis elegans, Turbatrix aceti) and phytoparasitic nematodes (Ditylenchus destructor). Working solutions were prepared in the following dilutions: 1) 1/1000, 2) 1/100, 3) 1/10, 4) 1/1, 5) undiluted, 6) control, water, and 7) standard (horseradish root juice). The solution of above concentrations was introduced into the plate wells of 0.5 mL in 4 repetitions, then a well-mixed nematode suspension of 0.5 mL was added with 50±10 nematodes in average. After 24 hours of hold-times, mobile and immobile nematodes were counted, then after 48 hours nematodes were counted again. At the end of the experiment, nematodes were washed from the drug in order to determine the nematostatic effect. In vitro screening showed that the horseradish root juice, black nightshade berry juice, and red nightshade leaf and berry juice had nematicidal properties. The nematostatic effect was recorded in shoots of the green tuber, tissues of the green surface of the tuber and tuber juices, juice of red nightshade leaves, and juice of black nightshade leaves and berries.