Hepatocellular Injury Ameliorated by a Common African Food, Parkia biglobosa

O. N. Onyekachi, S. Orji, C. Ugwu, C. Igwenyi, C. Uche, I. Abali, M. U. Nwobodo, C. Iwuoha, N. Chika-Igwenyi, C. A. Onyeaghala, F. Agu, A. I. Airaodion
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Abstract

Background: Parkia biglobosa seed has been reported to possess hepatoperotective potential. Therefore, this study sought to investigate its ability in ameliorating KBrO3-induced hepatotoxicity. Methodology: P. biglobosa was extracted with soxhlet extractor with 95% ethanol as the solvent. Twenty-four adult male Wistar rats were acclimatized under laboratory conditions and were randomly grouped into A, B, C and D. Group A was given distilled water orally. Animals in groups B, C and D were administered 100 mg/kg body weight of potassium bromate, but groups C and D were also treated with 100 and 200 mg/kg body weight of P. biglobosa respectively. Both potassium bromate and P. biglobosa were freshly prepared on daily basis and administered to rats by oral gavage. After 28 days of treatment, the animals were sacrificed under mild diethyl ether anaesthetization 24 hours after cessation of last treatment. Blood and liver tissue were collected. Results: The findings demonstrated that, when compared to the control group, KBrO3 caused a significant increase (P˂0.05) in ALT, AST, LDH, ALP, total bilirubin (TB), conjugated bilirubin (CB), and unconjugated bilirubin (UB) levels, but decreased total protein, albumin and globulin in the serum of animals. In the liver cells, KBrO3 reduced hepatic biomarkers. These perturbations were neutralized in the groups treated with 100 and 200 mg/kg body weight, respectively. Conclusion: The result of the present study revealed that KBrO3 is hepatotoxic at a dose of 100 mg/kg body weight. The result further suggests that P. biglobosa possesses hepatoprotective properties in rats in vivo. This study can be replicated in human trial.
一种常见的非洲食物对肝细胞损伤的改善作用
背景:据报道,大叶Parkia种子具有保肝潜力。因此,本研究旨在探讨其改善kbro3诱导的肝毒性的能力。方法:以95%乙醇为溶剂,用索氏提取器提取大黄花。在实验室条件下驯化24只成年雄性Wistar大鼠,随机分为A、B、C、d组,A组灌胃蒸馏水;B组、C组和D组分别给予100 mg/kg体重的溴酸钾,C组和D组分别给予100和200 mg/kg体重的大叶磷磷。每日新鲜配制溴酸钾和大鼠灌胃。治疗28 d后,末次治疗停止后24小时,以轻度乙醚麻醉处死。采集血液和肝脏组织。结果:与对照组相比,KBrO3使动物血清中ALT、AST、LDH、ALP、总胆红素(TB)、偶联胆红素(CB)和未偶联胆红素(UB)水平显著升高(P小于0.05),但使动物血清中总蛋白、白蛋白和球蛋白水平降低。在肝细胞中,KBrO3降低了肝脏生物标志物。在100和200 mg/kg体重组中,这些扰动被抵消。结论:本研究结果表明,KBrO3在100 mg/kg体重剂量下具有肝毒性。研究结果进一步表明,大叶枇杷在体内具有保护肝脏的作用。该研究可在人体试验中重复。
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