Genetic polymorphism of white mistletoe (Viscum album L.) in Ukraine

Faktori eksperimental'noi evolucii organizmiv Pub Date : 2021-08-31 DOI:10.7124/feeo.v28.1372

Yu. O. Bilonozhko, A. Rabokon, A. Postovoitova, L. Kalafat, N. S. Boĭko, S. M. Pryvalikhin, T. V. Topchii, A. Demkovych, Y. Blume, Y. Pirko

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Abstract

Aim. The aim of the study was to establish genetic differences between V. album growing in different parts of Ukraine. Methods. White mistletoe samples collected in different regions of Ukraine were used in the study. The method of estimating the intron length polymorphism of β-tubulin genes was used. Amplified DNA fragments were fractionated by non-denaturing polyacrylamide gel electrophoresis and visualized by silver nitrate staining. Results. The genotypes of 91 white mistletoe plants were analyzed. DNA profiles of white mistletoe with a specific amplicons of β-tubulin gene introns were obtained, which allowed to differentiate the samples from each other. Fingerprinting data were used for cluster analysis and dendrogram construction. Conclusions. It was found that the analyzed mistletoe samples did not differ by geographical factor and were characterized by a low level of genetic diversity in the studied samples. Keywords: Viscum album L., intron length polymorphism, β-tubulin, genetic variability, Ukraine.

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乌克兰白槲寄生的遗传多态性

的目标。这项研究的目的是建立在乌克兰不同地区生长的V. album之间的遗传差异。方法。研究中使用了在乌克兰不同地区收集的白槲寄生样本。采用β-微管蛋白基因内含子长度多态性估计方法。扩增的DNA片段经非变性聚丙烯酰胺凝胶电泳分离，硝酸银染色显示。结果。对91株白槲寄生植物进行了基因型分析。获得了具有β-微管蛋白内含子特异性扩增子的白槲寄生的DNA图谱，从而使样品能够相互区分。指纹图谱数据用于聚类分析和树状图构建。结论。结果表明，所分析的槲寄生样品不受地理因素的影响，具有遗传多样性较低的特点。关键词:Viscum album L.，内含子长度多态性，β-微管蛋白，遗传变异，乌克兰

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Faktori eksperimental'noi evolucii organizmiv

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