In vitro Activity of Picroside I in Type 2 Diabetes Based on Oxidative Stress

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY

Accounts of Chemical Research Pub Date : 2023-04-01 DOI:10.29356/jmcs.v67i2.1899

Jingya Liu, Yinqiu Zheng, Shuang Dai, Li Li, Wei Wu, R. Gou, Deyuan Wang, Shi Long, Meihua Huang, Z. Xu

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引用次数: 0

Abstract

Abstract. The primary factor leading to insulin resistance (IR) and type 2 diabetes mellitus (T2DM) is oxidative stress. Despite its liver-protecting, enzyme-lowering, immune-regulating, and antiviral effects, the impact of picroside I on oxidative stress, glucose utilization, and IR has not been investigated yet. In vitro studies were conducted to evaluate the antioxidant properties of different concentrations of picroside I. The results showed that picroside I effectively suppresses α-glucosidase and α-amylase with IC50 values of 109.75 μg/mL and 160.71 μg/mL in the range of 50-500 μg/mL. Additionally, when IR-HepG2 cells were treated with 80 μg/mL of picroside I, it was found to have little effect on cell viability, increase glucose consumption, decrease the levels of the free radical metabolite malonic dialdehyde, and increase superoxide dismutase activity. These findings indicate that picroside I has the potential to regulate oxidative stress in IR-HepG2 cells, potentially improving IR and exhibiting anti-T2DM activity. Resumen. El factor principal que conduce a la resistencia a la insulina (IR) y a la diabetes mellitus tipo 2 (T2DM) es el estrés oxidativo. A pesar de sus efectos protectores del hígado, reductores de enzimas, inmunorreguladores y antivirales, aún no se ha investigado el impacto del picrósido I sobre el estrés oxidativo, la utilización de glucosa y la IR. Se realizaron estudios in vitro para evaluar las propiedades antioxidantes de diferentes concentraciones de picrósido I. Los resultados mostraron que el picrósido I suprime eficazmente la α-glucosidasa y la α-amilasa con valores IC50 de 109,75 μg/mL y 160,71 μg/mL en el rango de 50 -500 microgramos/ml. Además, cuando las células IR-HepG2 se trataron con 80 μg/mL de picrósido I, se encontró que tenía poco efecto sobre la viabilidad celular, aumentaba el consumo de glucosa, disminuía los niveles del metabolito de radicales libres dialdehído malónico y aumentaba la actividad de la superóxido dismutasa. Estos hallazgos indican que el picrósido I tiene el potencial de regular el estrés oxidativo en las células IR-HepG2, mejorando potencialmente la IR y exhibiendo actividad anti-T2DM.

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基于氧化应激的picro苷I在2型糖尿病中的体外活性研究

摘要导致胰岛素抵抗(IR)和2型糖尿病(T2DM)的主要因素是氧化应激。尽管其具有保肝、降酶、免疫调节和抗病毒作用，但其对氧化应激、葡萄糖利用和IR的影响尚未被研究。结果表明，在50 ~ 500 μg/mL范围内，picro甙I能有效抑制α-葡萄糖苷酶和α-淀粉酶，IC50值分别为109.75和160.71 μg/mL。此外，80 μg/mL的picroside I处理IR-HepG2细胞时，发现对细胞活力影响不大，增加葡萄糖消耗，降低自由基代谢产物丙二醛水平，增加超氧化物歧化酶活性。这些发现表明，picroside I具有调节IR- hepg2细胞氧化应激的潜力，可能改善IR并表现出抗2型糖尿病的活性。El因子对胰岛素抵抗(IR)、2型糖尿病(T2DM)和应激氧化的影响。一项研究表明，通过抗病毒药物可以保护del hígado，还原enzimas，免疫调节，aún，目前还没有研究对del picrósido的影响，因为它可以抑制氧化，通过IR可以降低葡萄糖含量utilización。研究了不同浓度丙烯类抗氧化剂的体外对乙酰氨基葡萄糖苷(picrósido 1 . Los resultados mostraronque el picrósido 1) α-葡萄糖苷(α-amilasa con valores) IC50 de 109、75 μg/mL、160、71 μg/mL和50 ~ 500 μg/mL的乙酰氨基葡萄糖苷的体外对乙酰氨基葡萄糖苷(α-amilasa con valores)的体外对活性的影响。Además, cuando las csamulas IR-HepG2 se trataron con 80 μg/mL de picrósido I, se encontró que tenía poco effecto sobre la viabilidad cell, aumentaba el consumo de glucosa, disminuía los niveles del metabolito de radicales libres dialdehído malónico y aumentaba la actividad de la superóxido dismutasa。Estos hallazgos indican que el picrósido研究了正常应激细胞的氧化电位与正常应激细胞IR- hepg2的氧化电位、正常应激细胞IR- hepg2的氧化电位、正常应激细胞IR的活性及抗t2dm的作用。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.