Reduction of Some Heavy Metals in Fibre Cement Roofing Sheet Waste-Contaminated Soil by Consortium of Bacteria and Fungi

O. O. Akpomie, Catherine Ese Balogun, T. Akpomie
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Abstract

This research was carried out in order to ascertain the effectiveness of microbial remediation (bio-remediation) of environmental pollution by heavy metals from different sources in general and wastes from the manufacturing of fibre cement roofing sheets specifically. The concentrations of Cd, Cr, and Ni in fibre cement soil (0.11, 0.08 & 0.83), in dumpsite soil (4.17, 2.87 & 40.68) and in surrounding soil (2.11, 1.89 & 19.84) and soils outside the pollution area, control, (1.76, 0.89 & 14.17) mg/g respectively were determined using atomic absorption spectrophotometry. Preliminary results showed that the concentration of the heavy metals; Cd, Cr, and Ni were all higer than values recorded by the WHO/FEPA standard. Analysis of the variance of means between the heavy metals showed differences in the respective sampled soils (P= 0.209). The minimum inhibitory concentration (MIC) of the heavy metals on the test organisms of Bacillus sp, Rhizopus sp, proteus sp and microsporium canis were obtained by the Agar diffusion method from stock culture of isolates from fibre waste dumpsites at the Department of Microbiology, Delta State University Abraka. The MIC values for Ni on the respective test organisms were; 300, 150, 250 & 450, Cr; 250, 400, 350 & 450 while that for Cd was 900, 750, 900 & 700 μg/ml. Sterilized consortia of isolates inoculated with various combinations of bacteria and fungi were used to treat the experimental sampled soils. Concentrations of the respective heavy metals of the sampled soils were evaluated after the 1st and 12th week of treatments. Furthermore, the concentration of the respective heavy metals reduced in varying percentages between the 1st and 12th week of treatment and the results were also found to be significantly different statistically (t-calculated > t-critical). Overall, the percentage reduction in the heavy metal concentrations between the 1st and 12th week was higher in Cd and Cr (76.25% & 76.25%) respectively than in Ni (52.65%). This was an indication that microbial isolates were very effective in reducing heavy metals in fibre cement roofing sheet-wastes and from the environment.
利用细菌和真菌联合体减少纤维水泥屋面板废弃物污染土壤中的部分重金属
进行这项研究是为了确定微生物修复(生物修复)对来自不同来源的重金属环境污染的有效性,特别是来自纤维水泥屋面板制造的废物。采用原子吸收分光光度法测定了纤维水泥土(0.11、0.08和0.83)、垃圾场土壤(4.17、2.87和40.68)、周围土壤(2.11、1.89和19.84)和污染区外土壤、对照土壤(1.76、0.89和14.17)中Cd、Cr和Ni的浓度。初步结果表明:重金属浓度;Cd、Cr、Ni均高于WHO/FEPA标准。各样地重金属含量均值方差分析显示,各样地土壤重金属含量差异显著(P= 0.209)。采用琼脂扩散法从阿布拉卡三角洲州立大学微生物系的纤维废弃物填埋场分离物中获得芽孢杆菌、根霉、变形杆菌和犬小孢子的最低抑菌浓度(MIC)。各受试生物对Ni的MIC值为;300、150、250、450,Cr;Cd为900、750、900、700 μg/ml。用细菌和真菌的不同组合接种灭菌的分离菌群来处理实验样土。分别在处理第1周和第12周测定土壤样品中重金属的浓度。此外,在治疗第1周和第12周之间,各重金属浓度均有不同百分比的下降,结果也有统计学上的显著差异(t计算> t临界)。总体而言,第1 ~ 12周重金属浓度下降百分比Cd和Cr分别为76.25%和76.25%,高于Ni(52.65%)。这表明微生物分离物在减少纤维水泥屋面板废料和环境中的重金属方面非常有效。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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