Effect of the Aqueous Extract of Schumanniophyton magnificum Harms on Sexual Maturation and Fertility of Immature (K. schum) Female Rat

E. F. Bend, P. O. Oundoum, M. N. Njila, B. L. Koloko, Camille D. Nyonseu, S. Mandengue, P. Moundipa, T. Dimo, D. Lembè
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引用次数: 5

Abstract

The aim of the study was to bring scientific evidence of the curative action of the stem bark aqueous extract of Schumanniophyton magnificum (ASMa) on the sexual maturation and fertility of the immature female Wistar rat. Forty immature female rats were randomized and divided into 4 groups of ten animals each and orally treated with the ASMa at doses of 0 (distilled water), 200 mg/kg, 400 mg/kg and 800 mg/kg /BW/day for 30 consecutive days. Body weight and food intake were recorded throughout the experimental period. The precocity of the puberty onset in treated animals was evaluated through the determination of their age at vaginal opening. At the end of the experimental period, 5 animals in each group were sacrificed and blood samples were collected for hormonal assay. Their ovaries and uteri were removed, blotted, weighted and prepared for biochemical analysis. The remaining rats (5 per group) were crossed with males of proven fertility. After laparotomy (ten days after mating) and delivery, fertility and gestational parameters were recorded. It was noticed that, body weight gain increased significantly at all doses although there was no significant difference in food intake. The sexual maturation of treated animal was reduced to 5 days when compared to control. This was associated with the simultaneous elevation of FSH and LH (p mg/kg. However, the ovarian cholesterol significantly decreased (p mg/kg and 800 mg/kg. The animal treated at doses of 400 mg/kg and 800 mg/kg exhibited an early fertilization (2 - 3 days) when compared to the control one (9 - 14 days). The number of implantation site significantly increased (p mg/kg and 800 mg/kg) after laparotomy as well as the number of alive fetuses after delivery and gestational rate (80% and 100%) respectively. These results provide the strong evidence on the induction of the onset puberty and gonadotropin synthesis, the improvement of the ovarian folliculogenesis and the fertility effect of ASMa in immature rats.
黄花菜水提物对未成熟雌性大鼠性成熟和生育能力的影响
本研究旨在为黄花楸茎皮水提物(ASMa)对未成熟雌性Wistar大鼠性成熟和生育能力的治疗作用提供科学依据。将40只未成熟雌性大鼠随机分为4组,每组10只,分别以0(蒸馏水)、200 mg/kg、400 mg/kg和800 mg/kg /BW/d的剂量口服ASMa,连续30 d。记录试验期间的体重和摄食量。通过测定动物阴道开口时的年龄来评估治疗动物的性早熟。试验期结束时,每组处死5只动物,取血进行激素测定。切除卵巢和子宫,染色,称重,准备生化分析。其余大鼠(每组5只)与已证实生育能力的雄性杂交。剖腹手术(交配后10天)和分娩后,记录生育能力和妊娠参数。人们注意到,在所有剂量下,体重增加都显著增加,尽管食物摄入量没有显著差异。与对照组相比,治疗动物的性成熟时间缩短至5天。这与FSH和LH同时升高(p mg/kg)有关。卵巢胆固醇显著降低(p mg/kg和800 mg/kg)。与对照组(9 - 14天)相比,400mg /kg和800mg /kg剂量处理的动物表现出较早受精(2 - 3天)。剖腹手术后着床部位数(p mg/kg和800 mg/kg)、分娩后活胎数和妊娠率(80%和100%)均显著增加。这些结果为ASMa诱导未成熟大鼠青春期发育和促性腺激素合成,改善卵巢卵泡发生和生育作用提供了有力的证据。
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