α-Glycerophosphate dehydrogenase in the American cockroach, and its use as a phenetic tool in cockroaches

W.P Stephen, I.H Cheldelin
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引用次数: 5

Abstract

  • 1.

    1. Extracts from each of the fat body, thoracic and appendage muscle of both sexes of the American cockroach yielded a single disc electrophoretic GDH fraction, which was quantitative and qualitatively stable during the first 6 months of the imago.

  • 2.

    2. Enzymes extracts from 3 cockroach species were heat stable at 52° for 30 min.

  • 3.

    3. GDH patterns were highly species-specific among the 24 cockroach species analyzed. Generic relationships were postulated on the basis of Rf similarity.

  • 4.

    4. Clones of parthogenetic Pycnoscelus surinamensis, with modal chromosome complements of 34 to 54, each had a single GDH fraction and all Rf values were identical.

美洲蟑螂的α-甘油磷酸脱氢酶及其在蟑螂中作为遗传工具的作用
1.1. 从美洲蜚蠊的脂肪体、胸肌和附肢肌肉中提取的每一种提取液都产生了单个圆盘电泳GDH分数,该分数在图像的前6个月定量和定性上都是稳定的。3种蜚蠊酶提取物在52°温度下热稳定性为30 min。在分析的24种蟑螂中,GDH模式具有高度的种特异性。一般关系是基于Rf相似度假设的。附生的苏里南螺(Pycnoscelus surinamensis)的染色体模态互补数为34 ~ 54,每个克隆都有一个单一的GDH分数,所有Rf值相同。
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