Isolation, screening and characterization of gelatinase and protease producing microorganisms from organic kitchen waste

Abstract

The present study was aimed at isolation, screening and identification of gelatinase and protease producing bacteria from the kitchen waste. The study was also focused on the potential of isolates to produce enzyme protease and gelatinase. The total of 157 bacterial isolates were obtained from the organic kitchen waste collected from various nearby sources. The primary screening was done to screen the potential of these isolates for their enzymatic potentials on selective media. The 57 promising bacterial isolates were found to have enzymatic potentials for both gelatinase and protease. The screened isolates were subjected to secondary screening, amongst which five isolates showed maximum production of both gelatinase and protease. These five isolates viz. KW91, KW104, KW121, KW128, KW98 were the most promising isolates showing high degree of milk and gelatin hydrolysis which may be potent in faster degradation of domestic kitchen waste.