Relationship between CTLA4, TNF-α and PTPN22 gene polymorphism and the serum levels of antithyroglobulin and antiperoxidase antibodies in autoimmune thyroiditis

IF 0.4 Q4 MEDICINE, RESEARCH & EXPERIMENTAL
R. Rahimova
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引用次数: 1

Abstract

Autoimmune thyroiditis (AIT) is a chronic inflammatory that involves hyperactivation of the immune system against the thyroid gland, recognizing it as "nonself”. The aim of this research was to identify the relationships between genetic polymorphism in CTLA4, TNF-α and PTPN22 genes and the manifestation of AIT and levels of antithyroglobulin antibody (anti-TG Ab) and thyroid peroxidase antibody (anti-TPO Ab). The study was conducted during 2014–2020 and included 64 men and 106 women aged between 18 and 64 years with AIT. The control group consisted of 65 people (26 men, 39 women, aged between 20 and 65 years) without any thyroid pathologies or other autoimmune diseases. For molecular genetic analysis, real-time quantitative RT-PCR was used with fluorescently labeled FAM probes on a detection system CFX96 (BioRad). The results demonstrated that patients with the GG genotype and the G allele of the +49A/G polymorphism in the CTLA4 gene have significantly higher titers of anti-TG Ab. High titers of anti-TG Ab were detected in 22.4% of patients with the GG genotype (p = 0.005, χ2 = 7.86, OR = 0.237, 95% CI = 0.088–0.635), and in 55.6% of patients with the G allele (p = 0.0012, χ2 = 10.43, OR = 0.360, 95%, СI = 0.192–0.674). At the same time, the A allele of the +49A/G polymorphism is significantly more common in patients with normal anti-TG Ab values—in 68.1% of individuals (p = 0.0012, χ2 = 10.43, OR= 2.78, 95%, CI = 1.484–5.207). The results of the study indicate the prognostic significance of the G allele and the GG genotype of the +49A/G polymorphism of the CTLA4 gene predicting the probability of occurrence of anti-TG and anti-TPO antibodies.
自身免疫性甲状腺炎CTLA4、TNF-α和PTPN22基因多态性与血清抗甲状腺球蛋白和抗过氧化物酶抗体水平的关系
自身免疫性甲状腺炎(AIT)是一种慢性炎症,涉及免疫系统对甲状腺的过度激活,将其视为“非我”。本研究旨在探讨CTLA4、TNF-α和PTPN22基因多态性与AIT的表现及抗甲状腺球蛋白抗体(抗tg Ab)和甲状腺过氧化物酶抗体(抗tpo Ab)水平的关系。该研究于2014-2020年进行,包括64名男性和106名女性,年龄在18至64岁之间。对照组包括65人(26名男性,39名女性,年龄在20至65岁之间),无甲状腺疾病或其他自身免疫性疾病。在CFX96 (BioRad)检测系统上,荧光标记FAM探针采用实时定量RT-PCR进行分子遗传学分析。结果显示,GG基因型和CTLA4基因+49A/G多态性的G等位基因的患者抗tg抗体滴度明显较高,其中GG基因型患者的抗tg抗体滴度为22.4% (p = 0.005, χ2 = 7.86, OR = 0.237, 95% CI = 0.088 ~ 0.635), G等位基因型患者的抗tg抗体滴度为55.6% (p = 0.0012, χ2 = 10.43, OR = 0.360, 95%, СI = 0.172 ~ 0.674)。同时,+49A/G多态性的A等位基因在抗tg Ab值正常的患者中更为常见,占68.1% (p = 0.0012, χ2 = 10.43, OR= 2.78, 95%, CI = 1.484 ~ 5.207)。本研究结果提示CTLA4基因+49A/G多态性的G等位基因和GG基因型预测抗tg和抗tpo抗体发生概率的预后意义。
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来源期刊
AIMS Medical Science
AIMS Medical Science MEDICINE, RESEARCH & EXPERIMENTAL-
自引率
14.30%
发文量
20
审稿时长
12 weeks
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