RASSF1 and PTEN Promoter Hypermethylation Influences the Outcome in Epithelial Ovarian Cancer

Clinical Ovarian and Other Gynecologic Cancer Pub Date : 2014-12-01 DOI:10.1016/j.cogc.2014.12.002

Mariyam Zuberi , Rashid Mir , Sagar Dholariya , Imtiyaz Najar , Prasant Yadav , Jamsheed Javid , Sameer Guru , Masroor Mirza , Gauri Gandhi , Nita Khurana , Prakash C. Ray , Alpana Saxena

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引用次数: 11

Abstract

Background

To investigate the methylation pattern in promoter region of RASSF1A and PTEN genes in epithelial ovarian cancer patients in North India.

Patients and Methods

Fifty patients and 20 healthy controls were studied. Isolation of genomic DNA from peripheral blood and methylation-specific polymerase chain reaction (MSP) were applied for analysis.

Results

17 of 50 patients (34.0%) were found to be methylated for RASSF1A gene, whereas methylation of the PTEN gene occurred in 8 of 50 cases (16.0%). A statistically significant result was obtained (P = .01) for RASSF1A gene and correlated with the patients' clinicopathologic features.

Conclusion

Hypermethylation of both RASSF1A and PTEN genes in blood DNA from ovarian cancer patients might offer an exposition for early diagnosis of the malignancy.

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RASSF1和PTEN启动子超甲基化影响上皮性卵巢癌的预后

研究北印度上皮性卵巢癌患者RASSF1A和PTEN基因启动子区甲基化模式。患者与方法对50例患者和20例健康对照者进行研究。采用外周血基因组DNA分离和甲基化特异性聚合酶链反应(MSP)进行分析。结果50例患者中RASSF1A基因甲基化17例(34.0%)，PTEN基因甲基化8例(16.0%)。RASSF1A基因表达与患者临床病理特征相关，差异有统计学意义(P = 0.01)。结论卵巢癌患者血液DNA中RASSF1A和PTEN基因的高甲基化可能为恶性肿瘤的早期诊断提供线索。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Ovarian and Other Gynecologic Cancer

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