Utilidad de la separación magnética mediante columnas de anexina V en el procesado de muestras seminales para inseminación intrauterina

Medicina Reproductiva y Embriología Clínica Pub Date : 2016-12-01 DOI:10.1016/j.medre.2016.05.003

Marta Ballester , Montse Boada , Silvia Mateo , Rosario Buxaderas , Josep María Vendrell , Buenaventura Coroleu , Anna Veiga

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Abstract

Infertile men show a higher proportion of apoptotic spermatozoa in ejaculate, and have a negative impact on the assisted reproduction techniques (ART) results. An adequate sperm selection which eliminates the apoptotic spermatozoa could improve the results. The objective of the study was to evaluate the effect of eliminating apoptotic spermatozoa in the ejaculate by density gradient (DG) and Magnetic Activated Cell Sorting (MACS) processing of sperm on the pregnancy rate (PR) and new born rate (NBR) after intrauterine insemination (IUI).

A prospective observational and analytical study was performed on 566 cycles of IUI in 326 couples, from June 2012 to March 2014 in the Reproductive Medicine Service of Dexeus Women's Health comparing a study group (MACS) and a control group (Non-MACS). The preparation of the semen sample in both groups was by DG, and in the MACS group, the post capacitation fraction was processed using annexin V columns.

The PR and NBR obtained in MACS group were 16.2% and 12.9%, respectively versus 17.7% and 15.3% in Non-MACS group. No significant differences were observed.

Our results suggest that MACS used in IUI without a clinical indication does not improve the results in either the pregnancy rate or new born rate. The use of annexin V columns in other techniques, or specific indications cannot be ruled out. Further prospective randomised studies are needed in order to determine the advantages of MACS in ART.

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annexin V柱磁分离在宫内授精精精标本处理中的应用

不育男性在射精中出现较高比例的凋亡精子，对辅助生殖技术(ART)的效果产生负面影响。适当的精子选择可以消除凋亡的精子，从而改善结果。本研究旨在探讨密度梯度(DG)和磁活化细胞分选(MACS)处理对体外受精(IUI)后精子中凋亡精子的去除对妊娠率(PR)和新生儿出生率(NBR)的影响。对2012年6月至2014年3月在Dexeus妇女健康生殖医学服务中心进行的326对夫妇的566个IUI周期进行了前瞻性观察和分析研究，比较了研究组(MACS)和对照组(Non-MACS)。两组精液样品均采用DG制备，MACS组获能后部分采用膜联蛋白V柱处理。MACS组的PR和NBR分别为16.2%和12.9%，而非MACS组为17.7%和15.3%。未观察到显著差异。我们的研究结果表明，在没有临床指征的情况下，IUI中使用MACS并不能提高妊娠率和新生儿出生率。不能排除在其他技术或特定适应症中使用膜联蛋白V柱。为了确定MACS在ART中的优势，需要进一步的前瞻性随机研究。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Medicina Reproductiva y Embriología Clínica

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