Inhibiton of green okra fruit (Abelmochus esculentus) extract against Staphylococus aureus ATCC 6538 root canals of teeth

Oktavia Nur Vitasari, R. Nugroho, S. Lestari, Mahasiswa Kedokteran Gigi, Bagian Konservasi
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Abstract

Staphylococcus aureus is bacteria identified in the root canal of infected teeth by 20%, resistant and isolated from secondary root canal treatment. NaOCl 2.5%has toxic properties and causes irritation when pushed into periapical tissue. Green okra fruit (GOF) extract has antibacterial secondary metabolite compound i.e.flavonoids, alkaloids, saponins, tannins and terpenoids. This research was carried out to determine inhibition of GOF extract against S.aureus. Inhibition test useddisc diffution method which consisted of 5 research groups, i.e. GOF with concentration of 1.563%, 3.125%, 6.25%, and 12.5%, and 2.5% NaOCl; the ave-rage diameter of the inhibition zone of the concentration of GOF extract were 0 mm, 0 mm, 14.51 mm, 17.95 mm, and 24.56 mm, respectively. It was concluded that GOF extract has inhibition against of S.aureus starting from concentration of 6.25%. Green okra fruit extract has inhibition close to NaOCl 2.5% against S.aureus at concentration of 12.5%.
绿秋香果提取物对牙根管金黄色葡萄球菌ATCC 6538的抑制作用
金黄色葡萄球菌是在感染牙齿的根管中鉴定出20%的细菌,具有耐药性,从二次根管治疗中分离出来。NaOCl 2.5%具有毒性,当推入根尖周组织时引起刺激。绿秋葵果实(GOF)提取物具有抗菌次生代谢物化合物,即黄酮类化合物、生物碱、皂苷、单宁和萜类化合物。本研究旨在确定葡萄籽提取物对金黄色葡萄球菌的抑制作用。抑制试验采用圆盘扩散法,共设5个研究组,分别为浓度为1.563%、3.125%、6.25%、12.5%的GOF和2.5% NaOCl;GOF提取物浓度的平均抑菌带直径分别为0 mm、0 mm、14.51 mm、17.95 mm和24.56 mm。结果表明,GOF提取物对金黄色葡萄球菌的抑制作用从浓度为6.25%开始。绿秋葵果提取物对金黄色葡萄球菌的抑制作用接近2.5%,浓度为12.5%。
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