Synthesis and Characterization of Molecularly Imprinted Polymer for Prasugrel Drug Base on 2-Hydroxyethyl Methacrylate Monomer in Biological and Pharmaceutical Samples

Abstract

In this research work, a molecularly imprinted polymer (MIP) was synthesized for Prasugrel. In this method, a polymer was first synthesized using 2-hydroxyethyl methacrylate (HEMA) as a monomer, N, N’-methylenebisacrylamide as a crosslinker, and azobisisobutyronitrile (AIBN) as an initiator. The successful synthesis of the MIP was confirmed by elemental analysis (CHN), Fourier-transform infrared (FT-IR) spectroscopy and scanning electron microscopy (SEM). Then, the effects of various parameters such as pH, temperature, MIP capacity, selectivity, adsorption kinetics and adsorption isotherms were investigated by UV-Vis spectroscopy. The calibration curve was plotted for Prasugrel in these conditions and linear range, limit of detection (LOD) and limit of quantification (LOQ) were reported. Finally, the performance of the synthesized MIP as an adsorbent was studied under optimized conditions for the extraction and determination of Prasugrel in tablet matrix and biological fluids such as urine and plasma by high performance liquid chromatography (HPLC).