Development of micro-spectroscopic system and its application to single fibers

Abstract

A spectroscopic system that enables ordinary microscopes to measure spectra was developed. This system was applied to single ˆber analysis and successfully measured the micro-absorption spectra, polarized micro-absorption spectra, and micro-‰uorescence spectra. All the spectra showed good sensitivity and reproducibility, and the obtained spectra were substantially equivalent to those measured with commercially available instruments. By means of the developed system in this study, the color information of ˆbers can be obtained as spectra, which results in more precise and objective discrimination of ˆbers that have similar colors. This system can easily be attached to various existing microscopes without any modiˆcations, and will improve their performance.