Association of leptin gene polymorphism G-2548A with metabolic and anthropometric parameters in obese patients in a Serbian population: pilot study

Q Medicine

Clinical Lipidology Pub Date : 2014-10-01 DOI:10.2217/clp.14.42

Sanja Soskić, E. Stokic, M. Obradović, Emina Sudar, Nasta Tanić, A. Kupusinac, J. Djordjevic, E. Isenovic

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引用次数: 2

Abstract

Abstract Aim: The aim of this pilot study was to investigate possible associations of LEP promoter polymorphism LEP G-2548A and obesity-associated metabolic and antropometric parameters in obese population. Materials & methods: Group of 31 patients with hyperalimentary type of obesity (mean age: 39.26 ± 11.45 years; BMI: 41.51 ± 9.22 kg/m2) and 36 healthy, nonobese, normal weight subjects (mean age: 33.55 ± 6.46 years; BMI: 22.63 ± 1.94 kg/m2) were studied. Blood samples were collected for DNA isolation, serum leptin and serum lipids measurements. LEP G-2548A genotypes were determined by PCR restriction fragment length polymorphism based analyses. Results: No significant differences in genotype and allele frequencies of the LEP G-2548A polymorphism were detected between obese and normal weight subjects. No association was found between this polymorphism and BMI. Obese subjects had statistically significant increase in serum leptin levels compared with control, while no association between leptin concentration and LEP polymorphism LEP G-2548A was found. There is a statistically significant association of LEP G-2548A genotypes with LDL (p < 0.05), LDL/HDL ratio (p < 0.001), apoB (p < 0.01), body weight (p < 0.001) and waist circumference (p < 0.001). Conclusion: Our findings indicate that there is an association between LEP G-2548A polymorphism and metabolic and anthropometric parameters in obese patients in a Serbian population.

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瘦素基因多态性G-2548A与塞尔维亚人群肥胖患者代谢和人体测量参数的关联:初步研究

摘要目的:本初步研究的目的是探讨肥胖人群中LEP启动子多态性LEP G-2548A与肥胖相关的代谢和心率参数的可能关联。材料与方法:31例高消化型肥胖患者(平均年龄:39.26±11.45岁;BMI: 41.51±9.22 kg/m2)和36名健康、非肥胖、体重正常的受试者(平均年龄:33.55±6.46岁;BMI: 22.63±1.94 kg/m2)。采集血样进行DNA分离、血清瘦素和血脂测定。LEP G-2548A基因型采用PCR限制性片段长度多态性分析。结果:肥胖者与正常体重者LEP G-2548A多态性基因型及等位基因频率无显著差异。没有发现这种多态性与BMI之间的关联。肥胖组血清瘦素水平较对照组有统计学意义升高，而瘦素浓度与LEP多态性LEP G-2548A无相关性。LEP G-2548A基因型与LDL (p < 0.05)、LDL/HDL比值(p < 0.001)、apoB (p < 0.01)、体重(p < 0.001)、腰围(p < 0.001)有统计学意义。结论:我们的研究结果表明，LEP G-2548A多态性与塞尔维亚人群肥胖患者的代谢和人体测量参数之间存在关联。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Clinical Lipidology 生物-生化与分子生物学

CiteScore

0.44

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Clinical Lipidology is published to support the diverse array of medical professionals who work to reduce the incidence of morbidity and mortality from dyslipidemia and associated disorders of lipid metabolism. The Journal''s readership encompasses a broad cross-section of the medical community, including cardiologists, endocrinologists, and primary care physicians, as well as those involved in the treatment of such disorders as diabetes, hypertension, and obesity. The Journal also addresses allied health professionals who treat the patient base described above, such as pharmacists, nurse practitioners and dietitians. Because the scope of clinical lipidology is broad, the topics addressed by the Journal are equally diverse. Typical articles explore lipidology as it is practiced in the treatment setting, recent developments in pharmacological research, reports of treatment and trials, case studies, the impact of lifestyle modification, and similar academic material of interest to the practitioner. While preference is given to material of immediate practical concern, the science that underpins lipidology is forwarded by expert contributors so that evidence-based approaches to reducing cardiovascular and coronary heart disease can be made immediately available to our readers. Sections of the Journal will address pioneering studies and the clinicians who conduct them, case studies, ethical standards and conduct, professional guidance such as ATP and NCEP, editorial commentary, letters from readers, National Lipid Association (NLA) news and upcoming event information, as well as abstracts from the NLA annual scientific sessions and the scientific forums held by its chapters, when appropriate.