ISOLATION AND IDENTIFICATION OF ANTIOXIDANT CONSTITUENTS FROM EXTRACELLULAR METABOLITES OF LACTOBACILLUS BREVIS

S. Noureen, A. Riaz, E. Ali, I. Sajid, W. Zaman, N. Arshad
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Abstract

Oxidative stress is a metabolic condition characterized by excessive production of free radicals in the body. The condition leads to metabolic diseases including; aging, diabetes, atherosclerosis, myocardial infarction and cancer. Antioxidants are the mainstay to modulate the consequences of oxidative damage. Secondary metabolites from lactic acid producing bacteria possess antioxidant activity. The present study aimed at isolating and identifying antioxidant metabolites present in cell free supernatant (CFS) of broth culture of Lactobacillus brevis MG 000874. Thin layer chromatography (TLC) coupled bioautography of different fractions of methanol extract of CFS using 2, 2-diphenyl-1-picrylhydrazyl (DPPH) model indicated antioxidant activity in ethyl acetate fraction. Fourier transform infrared (FTIR) spectroscopy of the isolated compound revealed the presence of aldehyde and alkyl functional groups. GC-MS analysis led to identification of four active compounds viz. a) L-proline, N-valeryl, hexyl ester b) Ergotaman-3',6',18-Trione,12'-Hydroxy-2'-Methyl-5'-(2-Methylpropyl) c) 2-Methylthiolane, S, S-Dioxide and d) Phenol, 3,5-Bis(1,1-Dimethylethyl). Findings of present study may be useful in developing antioxidant drugs from bacterial origin.
短乳杆菌胞外代谢产物中抗氧化成分的分离与鉴定
氧化应激是一种代谢状况,其特征是体内自由基的过量产生。这种情况会导致代谢疾病,包括;衰老、糖尿病、动脉粥样硬化、心肌梗塞和癌症。抗氧化剂是调节氧化损伤后果的主要物质。乳酸菌的次生代谢物具有抗氧化活性。本研究旨在分离和鉴定短乳杆菌MG 000874肉汤培养的无细胞上清(CFS)中存在的抗氧化代谢物。采用2,2 -二苯基-1-苦酰肼(DPPH)模型对不同馏分的甲醇提取物进行薄层色谱(TLC)耦合生物自显像,结果表明乙酸乙酯馏分具有抗氧化活性。该化合物的傅里叶变换红外光谱(FTIR)显示出醛和烷基官能团的存在。GC-MS分析鉴定出4种活性化合物:a) l-脯氨酸,n-戊酰,己基酯;b)麦角甘烷-3',6',18-三酮,12'-羟基-2'-甲基-5'-(2-甲基丙基);c) 2-甲基硫烷,S, S-二氧化氮;d)苯酚,3,5-双(1,1-二甲基乙基)。本研究结果可能有助于开发细菌源抗氧化药物。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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