Investigation of Interaction of Soft Nanoparticles based Vesicles with Lung Surfactant via Langmuir-Blodgett trough and Quartz Crystal Microbalance Study

Abstract

Major aim of this research study is to comprehend the interaction of soft nanoparticle-based vesicles with model lung surfactant monolayer. Vertebrate lung surfactant plays a pivotal role in gaseous exchange plus breathing. As pulmonary lung surfactant is the one which directly comes in contact to inhaled air so it also acts as defensive agent against air-borne pollutants (any foreign particle). In this enquiry, several different types of lipids namely 1,2-dipalmitoyl-sn-glycero-3-phosphocholine, 1,2-Dimitrystoyl-sn-glycero-3-phosphorylcholine, 1-palmitoyl-2-oleoyl-sn-glyceero-3-phosphocholine are used as lipid monolayer and vesicles in different combinations while using Langmuir-Blodgett trough. The different lipids are treated with chloroform and buffer solution to make vesicles and then extruded. Nanoparticles present in vesicles interact with lipid monolayer when processed in Langmuir-Blodgett trough and mimics the behavior of natural pulmonary lung surfactant system. In Quartz Crystal with gold mounted on it as thin layer, different combinations of lipid bilayer and vesicles are used from the same list of lipids. The results obtained from this investigation are quite informative in depicting the interaction of soft nanoparticle based vesicles with lung surfactant system, also this work would pave the way to explore still more on interaction of soft nanoparticles based vesicles with lung surfactant, as very little work is done on soft nanoparticles. This novelty of this work is to plan to develop some alternatives of lung surfactant because we cannot perform the experiment directly in living souls as some of these has lethal effects.