Phytochemical screening and evaluation of the antioxidant and antibacterial activity of ‎Atriplex halimus from two regions Algeria (El Oued and Tlemcen)

GABJ Pub Date : 2021-05-15 DOI:10.46325/gabj.v5i2.72
T. Chaouche, F. Haddouchi, Fayza Abbou, M. Aissaoui, Ouhiba Boudjemai, Imane Ghellai, Souad Senhadji
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引用次数: 2

Abstract

The purpose of this work is to study the antioxidant and antibacterial activity of two plants of the same species « Atriplex halimus » from two different regions El Oued and Tlemcen. The plant leaves were subjected to a one-hour reflux extraction in methanol/acetone (70/30: v/v). Qualitative phytochemical examination of the leaf extract showed the presence of alkaloids, tannins and flavonoids of varying intensity. Coumarins, terpenoids and saponins are absent in both plants. Qualitative analysis of total polyphenols, flavonoids and condensed tannins of extracts shows that Atriplex halimus from the El Oued region has a high content of total polyphenols (10.25 ± 1.17 mg GAE/g DW) and tannins (9.23 ±1.09 mg EC/g DW) compared to Tlemcen. However, the high flavonoid content presented by Atriplex halimus from the Tlemcen region (3.09 ± 0.13 mg EC/g DW). The evaluation of the antioxidant activity of the extracts was carried out by three methods: total antioxidant capacity, trapping of the free radical DPPH and iron reducing power, the results obtained show that the extract of Atriplex halimus from Tlemcen reveals an interesting activity compared to that of El Oued with a CI50 = 193.47 ± 1.79µg/mL. Evaluation of antibacterial activity showing that El Oued extract has activity against Salmonella typhimirium ATCC 13311, Enterobacter cloacae ATCC 13047, Staphylococcus aureus ATCC 6538 and Listeria monocytogenes ATCC 15313 with a CMI value of 5 mg/mL, 10 mg/mL and 2.5 mg/mL respectively. However, Atriplex halimus from Tlemcen does not reveal any antibacterial activity against the strains tested.
阿尔及利亚两个地区(El Oued和Tlemcen)的halimus的植物化学筛选及抗氧化和抗菌活性评价
本研究的目的是研究来自El Oued和Tlemcen两个不同地区的同一物种“Atriplex halimus”的两种植物的抗氧化和抗菌活性。植物叶片在甲醇/丙酮(70/30:v/v)中回流提取1小时。叶提取物的定性植物化学检查显示存在不同强度的生物碱、单宁和黄酮类化合物。这两种植物都不含香豆素、萜类和皂甙。定性分析结果表明,El Oued地区的盐柳总多酚、总黄酮和缩合单宁含量均高于天青(10.25±1.17 mg GAE/g DW)和单宁(9.23±1.09 mg EC/g DW)。而来自特莱姆森地区的盐柳黄酮含量最高(3.09±0.13 mg EC/g DW)。通过总抗氧化能力、捕获自由基DPPH和铁还原能力三种方法对提取物的抗氧化活性进行了评价,结果表明,与El Oued相比,特勒姆森的Atriplex halimus提取物具有较好的抗氧化活性,其CI50 = 193.47±1.79µg/mL。抑菌活性评价表明,黄叶提取物对鼠伤寒沙门菌ATCC 13311、阴沟肠杆菌ATCC 13047、金黄色葡萄球菌ATCC 6538和单核增生李斯特菌ATCC 15313具有抑菌活性,CMI值分别为5 mg/mL、10 mg/mL和2.5 mg/mL。然而,来自特莱姆森的Atriplex halimus对所测试的菌株没有显示出任何抗菌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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