Stndy on diabetic rat therapy by islet transplantation with rat mesenchymal stem cells and scaffold of pig small intestinal submucosal

Abstract

Objective Engineer a scaffold with mesenchymal stem cells and small intestinal submucosal to evaluate the effect of islet transplantation in diabetic rats. Methods MSC and pancreatic islets were isolated from Sprague-Dawley rats, and SIS was isolated from Bamei pigs. MSC were seeded on the SIS to construct MSC-SIS scaffold. The STZ-induced diabetic rats were divided into three groups: islets, SIS, and MSC-SIS. The expressions of insulin and CD31 were detected by immunofluorescence on the 14th day after transplantation, and serum cytokines were detected by protein microarray.One-way ANOVA was used to compare the transplantation effect of each group. Results In MSC-SIS group, the expressions of insulin and CD31 were significantly higher than those in the other two groups. Cytokines of VEGFA were increased while TNF-α, IFN-γ and IL-6 decreased, showing a significant difference (P<0.05). These results suggest that MSC-SIS scaffold significantly improve graft function and promote the expression of insulin and CD31, which may be related to the angiogenesis and anti-inflammatory effects of MSC. Conclusions Mesenchymal stem cells combined with intestinal submucosal scaffold can improve the effect of islet transplantation and provide a new method for the treatment of diabetes. Key words: Islet transplantation; Mesenchymal stem cells; Small intestine; Stent