Chromotographic and wound healing studies of Jatropha curcas (Euphorbiacea)

Abstract

The wound healing properties of the methanol extract of the leaves of Jatropha curcas were studied by incision and excision wound models in rats. The wound healing effect was compared to that of the standard antibiotic, Cicatrin R . The histopathological profile, phytochemistry and the acute toxicity were also studied. An attempt was made to resolve the extract into its components using thin layer chromatography (TLC). Result of the incision and excision wound model shows significant (P Jatropha curcas . The prohealing action was due to increased collagen deposition as well as better alignment and maturation. The leaves of  Jatropha curcas were found to contain alkaloids, glycosides, saponins, flavonoids, steroids, proteins, tannins, reducing sugars, fats and oils. The intraperitoneal acute toxicity test (LD 50 ) of the extract in mice showed that it  as relatively safe, since at a dose of 5000 mg/kg there was no death. Among chromatographic solvent systems tested, chloroform:ethylacetate (6:4) gave the best resolution of the extract giving the highest number of spots. The present study demonstrates wound healing potential of Jatropha curcas Keywords : wound healing, Jatropha curcas, angiogenesis, epithelization, proliferation Journal of Pharmaceutical and Allied Sciences , Vol. 7 No. 2 (2010)