Effectiveness of genomic DNA extraction protocol from three species of African Mistletoe(Lorantheceae)

Abstract

Genomic DNA was isolated from the three different species of African Mistletoe parasitizing on 3 different trees. We attempted to study the expression of the â-tubulin gene in the leaves of these plant using ECO RI to target the â-tubulin gene in the genomic DNA. Results obtained revealed a fragment (2.1 Kbp) close to the documented size of the â-Tubulin gene (1.9 Kbp). The expression of the â-tubulin gene as revealed by ECO RI digestion was evident only during the wet season only, probably due to higher metabolic requirement of the parasitic plant during this season. There was very little expression of this gene fragment during the dry season.