INVESTIGATING LOW DOSE OF WEED MEDIATED SILVER NANOPARTICLES AGAINST PATHOGENIC BACTERIA

Abstract

Weeds are undesirable and unpleasant plants with robust growth. Around 18,000 weed species outcast native biodiversity and responsible for tremendous economic losses. Plants were used to treat numerous ailments since ancient times. Antimicrobial resistance (AMR) is an emerging global health problem, where approximately 700,000 people lose their life each year from AMR infections. Minimum inhibitory concentration of Biogenic silver nanoparticles against pathogenic bacterial strain has not been reported previously. This study aims to elucidate lowest biogenic Alkanna tinctoria mediated silver nanoparticles dose to treat bacterial infections. Qualitative phytochemical analysis performed using standard tests. The synthesized silver nanoparticles were characterized visually, UV–Vis spectroscopy and using X-ray diffraction. Minimum inhibitory concentration of biogenic silver nanoparticles was tested against gram positive (S. aureus) and gram-negative bacteria (E. coli). Alkanna tinctoria leave were positive for alkaloids, carbohydrate, saponins, amino acids, and fixed oil. The synthesized silver nanoparticles were in range of 60 to 73nm. Silver nanoparticles significantly affected E. coli as compared to S. aureus. The MIC for E. coli was recorded as 46.87 μg/mL and 93.75 μg/mL was effective against S.aureus. Search of naive plants as novel therapeutic agents along with nanotechnology can bring revolution in the field of drug development and medical sciences.