The identification of a cleaved amplified polymorphic sequence (CAPS) marker for the flax rust resistance gene M3

G. Hausner, K. Rashid, E. Kenaschuk, J. Procunier
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引用次数: 8

Abstract

A cleaved amplified polymorphic sequence (CAPS) marker has been developed for the flax rust resistance gene M3. This molecular marker was identified in a rust differential for M3 by screening primer sets, whose sequences were based on the nucleotide sequence of the M gene. One primer set was identified that co-amplified, along with other related sequences, a 2.9-kb DNA segment of M3 or an M-like gene tightly linked to the M3 locus. Restriction fragment analysis revealed several unique DNA fragments that were linked to the presence of the M3 gene in a F2 segregating population. This molecular marker along with recently developed markers for the L2, L6, L9, and L11 alleles will be useful for marker-assisted selection in breeding programs for obtaining multigenic resistance against flax rust in Canadian flax cultivars.
亚麻抗锈病基因M3的裂解扩增多态性序列(CAPS)标记
建立了亚麻抗锈病基因M3的CAPS标记。根据M基因的核苷酸序列筛选引物,在M3锈病鉴别中鉴定出该分子标记。其中一组引物与其他相关序列一起扩增了M3的2.9 kb DNA片段或与M3位点紧密相连的m样基因。限制性内切酶片段分析显示,在F2分离群体中,有几个独特的DNA片段与M3基因的存在有关。该分子标记与最近开发的L2、L6、L9和L11等位基因标记将有助于标记辅助选择,以获得加拿大亚麻品种的多基因抗锈病育种计划。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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