Comparative Occurrence of TLR3 and TLR7 Polymorphisms among Healthy Individual of Various Population

Q4 Pharmacology, Toxicology and Pharmaceutics

Current Pharmacogenomics and Personalized Medicine Pub Date : 2021-06-28 DOI:10.2174/1875692118666210628122443

HariOm Singh, Vijay Chauware, Kamini Jakhar

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Abstract

Toll-like receptors (TLRs) act as mediators of an innate immune response to infectious agents. The risk for chronic infection and the development of cancer can be potentially influenced by the genetic variations in the TLR genes. TLR3 and TLR7 genes have been associated with susceptibility to several infections and immune diseases. Human population is very diverse. There are significant variations in the TLR3 and TLR7 polymorphisms among ethnic groups. Variations within the population are associated with the disease outcome. Hence, we aimed to compare the occurrence of TLR3 (rs5743312 C/T, rs3775296 C/A, and rs3775291 C/T) and TLR7 (rs179009 and rs179008) polymorphisms among healthy individuals of various populations. Genotyping TLR3 (rs5743312 C/T, rs3775296 C/A, and rs3775291 C/T) and TLR7 (rs179009 and rs179008) polymorphisms were done in 158 healthy controls from Western India by utilization of PCR-RFLP. In our study population, the prevalence of TLR3 rs5743312 CC, CT, and TT genotypes were found to be 67.1%, 31.0%, and 1.9%, respectively, whereas genotype distribution of rs3775296 C/A polymorphism was 65.2%, 31.6%, and 3.2%, respectively, and rs3775291C/T was 59.5%, 32.3% and 8.2%, respectively. The occurrence of TLR7 rs179008AA, rs179008AT, rs179008TT genotypes and rs179008A, rs179008T alleles in the healthy individuals were found to be 81.0%, 16.5%, 2.5% and 89.24%, 10.75%, respectively. The prevalence of TLR7 rs179009AA, rs179009AG, rs179009GG genotypes and rs179009A, rs179009G alleles in healthy individuals were 63.3%, 29.1%, 7.6% and 63.3%, 36.7%, respectively. The frequency of TLR7 polymorphism was compared with Italian, Asian, European, African, German, and France populations. The frequency of TLR3 polymorphism was compared with Asians, Caucasians, Taiwanese, Caucasians and Saudi Arabians, Poland, Taiwanese, Italy, Taiwan, Estonia, Asia, and the Caucasus. The inter-population differences were observed in the distribution of TLR3 and TLR7 polymorphisms. The prevalence of TLR3 and TLR7 polymorphisms suggested that genotype-phenotype studies should be conducted among population to address the innate immune responses against pathogens.

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不同人群健康个体TLR3和TLR7多态性的比较

toll样受体(TLRs)作为对感染因子的先天免疫反应的介质。慢性感染和癌症发展的风险可能受到TLR基因遗传变异的潜在影响。TLR3和TLR7基因与几种感染和免疫疾病的易感性有关。人口是非常多样化的。TLR3和TLR7基因多态性在不同民族间存在显著差异。人群中的变异与疾病的结果有关。因此，我们旨在比较不同人群健康个体中TLR3 (rss5743312 C/T、rs3775296 C/A和rs3775291 C/T)和TLR7 (rs179009和rs179008)多态性的发生情况。利用PCR-RFLP方法对158名来自印度西部的健康对照进行了TLR3 (rss5743312 C/T、rs3775296 C/A和rs3775291 C/T)和TLR7 (rs179009和rs179008)多态性的基因分型。在我们的研究人群中，TLR3 rs5743312 CC、CT和TT基因型的患病率分别为67.1%、31.0%和1.9%，而rs3775296 C/A多态性的基因型分布分别为65.2%、31.6%和3.2%，rs3775291C/T基因型分布分别为59.5%、32.3%和8.2%。健康人群中TLR7 rs179008AA、rs179008AT、rs179008TT基因型和rs179008A、rs179008T等位基因的发生率分别为81.0%、16.5%、2.5%和89.24%、10.75%。健康人群中TLR7 rs179009AA、rs179009AG、rs179009GG基因型和rs179009A、rs179009G等位基因的患病率分别为63.3%、29.1%、7.6%和63.3%、36.7%。比较了意大利、亚洲、欧洲、非洲、德国和法国人群中TLR7多态性的频率。比较了亚洲人、高加索人、台湾人、高加索人和沙特阿拉伯人、波兰人、台湾人、意大利人、台湾人、爱沙尼亚人、亚洲人和高加索人的TLR3多态性频率。群体间TLR3和TLR7多态性分布存在差异。TLR3和TLR7多态性的普遍存在表明，应该在人群中进行基因型-表型研究，以解决对病原体的先天免疫反应。

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来源期刊

Current Pharmacogenomics and Personalized Medicine Pharmacology, Toxicology and Pharmaceutics-Pharmacology

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0.40

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0.00%

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期刊介绍： Current Pharmacogenomics and Personalized Medicine (Formerly ‘Current Pharmacogenomics’) Current Pharmacogenomics and Personalized Medicine (CPPM) is an international peer reviewed biomedical journal that publishes expert reviews, and state of the art analyses on all aspects of pharmacogenomics and personalized medicine under a single cover. The CPPM addresses the complex transdisciplinary challenges and promises emerging from the fusion of knowledge domains in therapeutics and diagnostics (i.e., theragnostics). The journal bears in mind the increasingly globalized nature of health research and services.