Suppressive Effect In Vitro of Magnesium l-Ascorbyl-2-phosphate (APM), an Ascorbic Acid Derivative, on the Impairment of DNA by Glucose-6-phosphate

Abstract

We attempted to find compounds that suppress the DNA impairment caused by d-glucose-6-phosphate (Glc-6-P) by observing the loss of ability of pBR322 to transform Escherichia coli, and found that APM (magnesium l-ascorbyl-2-phosphate) suppressed the loss of transformability to less than 1/100 of that in the absence of APM. Wiien 2′-deoxyguanosine 5′-monophosphate (dGMP) was incubated in the dark with Glc-6-P and APM, changes in their absorbance patterns were observed, indicating possible suppression by APM of the interactions between dGMP and Glc-6-P. APM weakly suppressed the Maillard reaction.