ELECTROSPUN GELATIN NANOFIBROUS SCAFFOLDS – APPLICATIONS IN TISSUE ENGINEERING

Abstract

Electrospinning is highly used technique in the tissue engineering field, particularly in biomedical application [1]. The constricted concepts of this process are based on generate nonwoven nanofibers. The method involves high voltage electricity which is applied to the liquid solution and a collector, which lets the solution force out from a nozzle forming a jet. The jet formed fibers under influence of electrostatic forces concentrated and deposited these on the collector. Main objective of this study was to fabricate gelatin scaffolds with micro/nano-scale for successful wound dressing. Gelatin can mimic the chemical composition, physical structure and structure of the native skin extracellular matrix (ECM). However, the first and main principle in this study is the optimization of parameters of the electrospinning process. The used parameters have a crucial role in obtaining suitable fibers for further cell seeding and cell growth in vitro. With the use of series of various biocompatible polymers and solvents, solutions were tested in various electrospinning settings in order to produce microscale fibers. The scaffolds were analysed with scanning electron microscope images for fiber diameter measurement.