TOPICAL APPLICATION OF TERMINALIA CHEBULA CREAM INHIBITS ULTRAVIOLET-B-INDUCED PSORIASIS IN RATS

Aruna Pai, J. Rao, Madhu J. Rajendra, Ashish Jain
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Abstract

Introduction: The present study aims at evaluating anti oxidant activity and inhibition of topical application of Terminalia chebula cream in ultraviolet –B-induced psoriasis in rats. Materials &Methods: The seeds of Terminalia chebula were dried and powder was extracted with chloroform, ethyl acetate, ethanol, methanol, and deionized water by cold maceration method. Total phenolic content, total tannin content and total triterpenoid content was determined by different methods. Anti oxidant activity was evaluated by DPPH method and reducing power assay. Cream formulation of the optimized extract was prepared by hot fusion method. Psoriasis was induced by irradiatiating for 30 min at a vertical distance of 20 cm with UV light (385 nm) on the skin. Severity index, hydroxyproline content and epidermal thickness was determined. Results and Discussion: Total Phenol (mg gallic acid/ g) content, Total Tannins (mg catechin/ g) content and  Total triterpenoid (mg ursolic acid/ g) content was found to be significant for water extract.The reducing ability of water extract was always higher than methanol extract at all the concentrations. Conclusion: We concluded that cream containing 0.1% water extract of Terminalia chebula seeds possess antioxidant activity along with potent antipsoriatic activity. Further, application of Terminalia chebula cream resulted in decrease in relative epidermal thickness of animal skin as well as other histopathological features. The present investigation aims at the development of potent phytomedicine for treatment of psoriasis from the Terminalia chebula plant.
局部应用桔黄乳膏抑制紫外线b诱导的大鼠银屑病
摘要:本研究旨在评价慈颜膏对紫外光- b诱导的银屑病大鼠的抗氧化活性和抑制作用。材料与方法:用氯仿、乙酸乙酯、乙醇、甲醇、去离子水冷浸法对大黄种子进行干燥提取。用不同的方法测定总酚含量、总单宁含量和总三萜含量。采用DPPH法和还原力法评价其抗氧化活性。采用热熔法制备最佳提取液的乳膏配方。采用385 nm紫外光照射皮肤,垂直距离20 cm照射30 min,诱导牛皮癣。测定其严重程度指数、羟脯氨酸含量和表皮厚度。结果与讨论:水提物中总酚(没食子酸mg / g)含量、总单宁(儿茶素mg / g)含量和总三萜(熊果酸mg / g)含量具有显著性。在不同浓度下,水提物的还原能力均高于甲醇提物。结论:含0.1%慈母种子水提物的乳膏具有抗氧化活性和抗银屑病活性。此外,应用chebula乳膏导致动物皮肤的相对表皮厚度减少以及其他组织病理学特征。本研究旨在开发治疗银屑病的强效植物药。
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