STEREOLOGICAL EVIDENCE FOR DE/RE-GENERATION OF MYELIN SHEATHS IN AGED BRAIN WHITE MATTER OF FEMALE RATS

IF 0.8 4区计算机科学 Q4 IMAGING SCIENCE & PHOTOGRAPHIC TECHNOLOGY

Image Analysis & Stereology Pub Date : 2017-06-23 DOI:10.5566/IAS.1436

Chen Li, Lei Zhang, Qiaoya Ma, Yong Tang, Ya He

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引用次数: 4

Abstract

Studies have provided qualitative evidence of de-myelination and re-myelination in aged brain white matter. However, there have been no quantitative evidences of degeneration and regeneration of myelin sheaths in white matter. The present study was designed to investigate the quantitative changes in myelin sheaths using unbiased stereological techniques and qualitative changes using electron microscopy in aged brain white matter. Results obtained showed that in brain white matter, the total volume of myelin sheaths of old-age female rats was not significantly different from that of young female rats, but the total length of myelinated fibers in old female rats was significantly decreased by 46.1% when compared with that of young female rats. Myelin sheath volume per unit length of myelinated fibers of old female rats was significantly increased by 43.4% compared with that of young female rats. The mean thickness of myelin sheaths in the white matter of the old rats was significantly increased by 33.3%, when compared with that of young female rats. In age-related loss of myelinated fibers, most fibers had diameters less than 1.4 μm, and myelin sheath thicknesses less than 0.14 μm, but the length of myelinated fibers with diameters more than 0.6 μm and myelin sheath thicknesses more than 0.22 μm increased with age. Myelinated fibers with ratios of myelin sheath thicknesses to myelinated fiber external diameter less than 0.21 were significantly lower in elderly rats than in young rats. However, the total length of myelinated fibers with ratios of myelin sheath thicknesses to myelinated fiber external diameter more than 0.23 was higher in aged rats than in young rats. About 6.58% of myelin sheaths showed degenerative alterations, while 0.88% myelin sheaths showed regenerative alterations. This study provides stereological evidence not only for degeneration but also regeneration of myelin sheaths in aged white matter.

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雌性大鼠老龄脑白质髓鞘脱除/再生的体视学证据

研究提供了老年脑白质脱髓鞘和再髓鞘形成的定性证据。然而，没有定量的证据表明髓鞘在白质中退化和再生。本研究旨在利用无偏体视技术研究老年脑白质髓鞘的定量变化和电子显微镜下的质变。结果表明，老年雌性大鼠脑白质髓鞘总量与年轻雌性大鼠无显著差异，但老年雌性大鼠髓鞘总长度较年轻雌性大鼠明显减少46.1%。老龄雌性大鼠单位长度髓鞘体积比年轻雌性大鼠显著增加43.4%。老龄大鼠脑白质髓鞘平均厚度较年轻雌性大鼠显著增加33.3%。在年龄相关性的髓鞘纤维损失中，大多数纤维直径小于1.4 μm，髓鞘厚度小于0.14 μm，但直径大于0.6 μm的髓鞘长度和髓鞘厚度大于0.22 μm的髓鞘长度随着年龄的增长而增加。老年大鼠髓鞘厚度与髓鞘纤维外径之比小于0.21的有髓纤维明显低于年轻大鼠。老年大鼠髓鞘厚度与髓鞘纤维外径之比大于0.23的有髓纤维总长度高于幼年大鼠。约6.58%的髓鞘发生退行性改变，0.88%的髓鞘发生再生改变。本研究不仅为老年白质髓鞘的退化和再生提供了体视学证据。

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来源期刊

Image Analysis & Stereology MATERIALS SCIENCE, MULTIDISCIPLINARY-MATHEMATICS, APPLIED

CiteScore

2.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Image Analysis and Stereology is the official journal of the International Society for Stereology & Image Analysis. It promotes the exchange of scientific, technical, organizational and other information on the quantitative analysis of data having a geometrical structure, including stereology, differential geometry, image analysis, image processing, mathematical morphology, stochastic geometry, statistics, pattern recognition, and related topics. The fields of application are not restricted and range from biomedicine, materials sciences and physics to geology and geography.