Artem S. Silchenko , Huynh Hoang Nhu Khanh , Cao Thi Thuy Hang , Valeriya V. Kurilenko , Alexander M. Zakharenko , Anastasiya O. Zueva , Bui Minh Ly , Mikhail I. Kusaykin
{"title":"A Simple Plate Method for the Screening and Detection of Fucoidanases","authors":"Artem S. Silchenko , Huynh Hoang Nhu Khanh , Cao Thi Thuy Hang , Valeriya V. Kurilenko , Alexander M. Zakharenko , Anastasiya O. Zueva , Bui Minh Ly , Mikhail I. Kusaykin","doi":"10.1016/j.als.2015.12.002","DOIUrl":null,"url":null,"abstract":"<div><p>A novel method was developed for the screening and detection of fucoidanase activity in bacterial colonies using fucoidan–agarose plates and staining with hexadecyltrimethylammonium bromide (Cetavlon). Colonies with fucoidanase activity were indicated by a transparent halo. The medium containing undegraded fucoidan showed as a milk-colored background, but areas with degraded fucoidan under the colonies were visualized as a transparent halo.</p></div>","PeriodicalId":100012,"journal":{"name":"Achievements in the Life Sciences","volume":"9 2","pages":"Pages 104-106"},"PeriodicalIF":0.0000,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.als.2015.12.002","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Achievements in the Life Sciences","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2078152015000541","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5
Abstract
A novel method was developed for the screening and detection of fucoidanase activity in bacterial colonies using fucoidan–agarose plates and staining with hexadecyltrimethylammonium bromide (Cetavlon). Colonies with fucoidanase activity were indicated by a transparent halo. The medium containing undegraded fucoidan showed as a milk-colored background, but areas with degraded fucoidan under the colonies were visualized as a transparent halo.